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| Identifier |
000376765 |
| Title |
Regulation of Ets2 repressor factor by Homeodomain Interacting Protein Kinase 1 |
| Alternative Title |
Ρύθμιση του Ets2 από την Homeodomain Interacting Protein Kinase 1. |
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Author
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Γιαπιτζάκη, Γιολάντα Γεω.
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Thesis advisor
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Μαυροθαλασσίτης, Γεώργιος
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| Abstract |
Ets2 repressor factor (ERF) belongs to a family of transcription factors characterized by an evolutionary conserved Ets DNA binding domain. The Ets2 repressor factor is a ubiquitously expressed factor with tumor suppressor activity regulated by the Ras/Erk signaling pathway. ERF is phosphorylated by ERKs, which affects its sub‐cellular localization and is also involved in cell cucle progression through G1. A yeast‐two hybrid screen identified homeodomain interacting protein kinase 1 (HIPK1) as a potent Erf interactant protein. HIPK1 belongs to a family of four serine/threonine kinases (HIPK1‐4) highly conserved in vertebrates. Their localization in the nucleus has a speckled organization and these structures are likely involved in signalling mechanisms together with colocalizing proteins. HIPK1 also show a dynamic distribution in the sub‐nuclear compartments depending on the cellular conditions. Immunoprecipitation assays confirmed the interaction between HIPK1 and ERF. The ERF‐HIPK1 interaction is mapped at the amino‐terminal part of the Erf protein and results in ERF phosphorylation in vitro. Here, we sought to identify the HIPK1‐ ERF interaction domain and functional correlation of the two proteins. The interaction domain of
HIPK1 is mapped at the carboxy‐terminal part of the protein. Furthermore immunostainings assays
were performed to view the subcellular localization of HIPK1 truncated proteins and finally we tested
whether HIPK1 affects ERF mediated transcriptional repression.
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| Language |
Greek, English |
| Subject |
Developmental biology |
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Immunoprecipitation |
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Molecular biology |
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Ανοσοκατακρήμνιση |
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Κίναση |
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Μοριακή βιολογία |
| Issue date |
2012-11-16 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
275 |
Regulation of Ets2 repressor factor by Homeodomain Interacting Protein Kinase 1
