|
|
| Identifier |
uch.chemistry.msc//2004papadioti |
| Title |
Μελέτη της σχέσης της σωματοστατίνης και του μονοξειδίου του αζώτου στον αμφιβληστροειδή ποντικού |
| Creator |
Papadioti, Anastasia
|
| Abstract |
Somatostatin is a neuropeptide widely distributed in the central and peripheral nervous system. It mediates a diverse number of physiological actions by interacting with specific receptors in the plasma membrane, namely SSTR1-5. These receptors belong to the family of G protein-coupled receptors and can couple to diverse signal transduction pathways. Somatostatin and its receptors are found in the retina. Recent evidence suggested the involvement of SSTR2A and SSTR2B receptors in the regulation of nitric oxide (Vasilaki et al., 2001). Subsequent studies showed that somatostatin increased nitric oxide production by an SSTR2 mechanism (Vasilaki et al., 2002). In the present study, we examined first the relationship of somatostatin and nitric oxide in the retina of wild type mice. Subsequently, we used somatostatin deficient mice in order to substantiate the role of somatostatin on NO physiology. Immunohistochemical studies were performed employing polyclonal antibodies against SSTR2A and nNOS, the neuronal isotype of the nitric oxide synthase. NADPH-diaphorase was assessed histochemically. Double labeling experiments of NADPH-diaphorase staining with SSTR2A receptor, as well as with markers of retinal cell types (PKC and MAP-1A) were performed. A morphometric study of retinal sections stained with NADPH-diaphorase was performed to detect possible differences between retinas of wild type and somatostatin deficient mice. Different ages of pairs of animals were examined. Our results showed that the SSTR2A receptor was colocalised with NADPH-diaphorase in rod bipolar cells of the mouse retina. nNOS was detected only in amacrine cells suggesting the presence of the other enzyme isoforms in the mouse retina. Morphometry measurements of retinas stained with NADPH-diaphorase showed that the thickness of the retinas was increased in somatostatin deficient mice as compared to wild type mice (age 0.5months). This increase was not observed in adult mice. These findings suggest that somatostatin acting through SSTR2A receptors may affect nitric oxide production in the mouse retina.
|
| Subject |
σωματοστατίνη, ΝΟ, αμφιβληστροειδής, ανοσοϊστοχημεία, NADPH-διαφοράση, ποντίκια με έλλειψη του γονιδίου σωματοστατίνης,somatostatin, NO, retina, immunohistochemistry, NADPH-diaphorase, somatostatin deficient mice |
| Issue date |
2004-11-01 |
| Date available |
2007-02-06 |
| Collection
|
School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
|
|
|
Type of Work--Post-graduate theses
|
| Views |
188 |
Μελέτη της σχέσης της σωματοστατίνης και του μονοξειδίου του αζώτου στον αμφιβληστροειδή ποντικού
