E-Locus - Institutional Repository of the University of Crete - Συμπεριφορική και ιστολογική μελέτη στο μοντέλο του τραυματισμού του νωτιαίου μυελού μετά από μεταμόσχευση τρισδιάστατα καλλιεργούμενων νευρικών βλαστικών κυττάρων.

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Identifier

000408525

Title

Συμπεριφορική και ιστολογική μελέτη στο μοντέλο του τραυματισμού του νωτιαίου μυελού μετά από μεταμόσχευση τρισδιάστατα καλλιεργούμενων νευρικών βλαστικών κυττάρων.

Alternative Title

Behavioral and histological study in a model of spinal cord injury after the transplantation of 3D cultured neural stem cells.

Author

Κυριακίδου, Μαρία

Thesis advisor

Χαραλαμπόπουλος, Ιωάννης

Reviewer

Βενυχάκη, Μαρία
Σιδηροπούλου, Κυριακή

Abstract

Every year, around the world, 250,000-500,000 new cases of spinal cord injury (SCI) occur. Despite the severe health, social and economic consequences of SCI, a successful therapy for the neurological damage after SCI does not exist. Unlike the peripheral nervous system (PNS), the central nervous system (CNS) does not regenerate after injury, and recently a series of factors due to which there is lack of regeneration have been identified. The environment of the injury in the spinal cord contains inhibitory factors for the regeneration and the growth of the severed axons. These factors include cysts and the glial scar, inhibitory molecules which are associated with the glial scar and the myelin, and also the lack of necessary neurotrophic factors. In the present study, the therapeutic approach of the transplantation of 3D cultured Neural Stem Cells (NSCs) in a SCI model in mice has been studied. NCSs are able to differentiate into neurons and glial cells, and these cells can undertake the function that the damaged tissue had. Furthermore, these cells produce and secrete neurotrophins that can facilitate the survival of the injured neurons and also increase the regrowth of the axons. Additionally, the special collagen scaffold in which the cells are transplanted, fills the cavities caused by the injury and decreases the formation of the glial scar. In order to test the effectiveness of this therapeutic effort, the movement capacity of the animals that did or did not get the treatment was evaluated, in combination with histological analysis. The walking of the mice was tested with the Ladder Rung Walking test and it has been found to be improved in the group in which the animals got the transplant in comparison with the injury group. This observed positive trend requires further and thorough study in cellular and molecular level, in order to be confirmed and the exact cellular mechanisms involved in the processing of this protective action to be described.

Language

Greek

Issue date

2017-03-29

Collection

School/Department--School of Medicine--Department of Medicine--Post-graduate theses

Type of Work--Post-graduate theses

Views

205

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