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Identifier

000403665

Title

A study of sequence and structural constraints in the bidirectional promoters of S. cerevisiae

Alternative Title

Μελέτη των περιορισμών της αλληλουχίας και δομής των αμφίδρομων υποκινητών στο γονιδιώματα του S. cerevisiae

Author

Παπαζωγονόπουλος, Διόνυσος Σ.

Thesis advisor

Νικολάου, Χριστόφορος

Reviewer

Ηλιόπουλος, Ιωάννης
Σπηλιανάκης, Χαράλαμπος

Abstract

Bidirectional gene pairs, also known as head-to-head gene pairs, are defined as two genes on different DNA strands with adjacent 5'-ends that are transcribed divergently. Their transcription is often coordinated in order to achieve their biological role. The region between a bidirectional gene pair is designated as a putative bidirectional promoter, which is suggested to coordinately regulate the expression of the pair. Given that divergently transcribed genes constitute quite a substantial percentage of the total genes in a wide range of organisms, many studies have already focused on the characteristics of bidirectional promoters. In this study, we focused our work on bidirectional promoters of S. cerevisiae from the point of view of sequence and structural constraints. We performed various types of analyses that included data for the phylogenetic conservation of sequences, the robustness of symmetry of DNA curvature, as a mark of structural information, data from nucleosome maps to quantify nucleosomal occupancy and, lastly, data of single nucleotide polymorphisms (SNPs). With the use of all that, we have tried to discover and describe the inherent sequential and structural elements that distinguish bidirectional intergenic regions and promoters from the intergenic regions of other possible gene pair organizations, in order to draw conclusions on the underlying mechanisms and constraints that define the regulation and diversification of bidirectional transcription in a simple eukaryotic genome.

Language

English

Subject

Genome organization

Intergenic regions

Yeast

Αμφίδρομοι υποκινητές

Ζύμη

Οργάνωση γονιδιώματος

Issue date

2016-11-18

Collection