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Identifier

uch.biology.msc//2003pliakoni

Title

Αποτελεί η επαγωγή έκφρασης του Vvgdha και η αύξηση της ενεργότητας της Γλουταμικής Αφυδρογονάσης μηχανισμό αντίδρασης στην Αλατότητα;

Creator

Pliakoni, Eleni

Abstract

Glutamate dehydrogenase (GDH, EC 1.4.1.2) catalyses the bidirectional reaction of the reductive amination of α-ketoglutarate to glutamate in the mitochondria, though its precise physiological role hasnt been determined. One important abiotic stress factor in plants is salinity. Salinity is a major stress, which incorporates osmotic and ionic stress, as well as secondary stresses, such as the over-production of reactive oxygen species (ROS), which is termed oxidative stress. In this work the possible role of GDH while under salinity stress was investigated. In particular the production of ROS was studied, under NaCl stress, as well as the induction of the GDH transcript and specific activity. Grapevine and tobacco leaf discs, as well as calli and grapevine cell suspension cultures. Salinity stress led to the critical accumulation of ROS expression of the Vvgdha gene, accumulation of the anodic isoezymes and synthesis of the a-subunit of GDH, as well as the significant increase of the GDH specific activity. Nevertheless in the NaCl tolerant grapevine genotype GDH was not induced despite the accumulation of ROS, which prevents the establishment of a direct correlation between the induction of ROS and GDH. Possibly ROS indirectly induce GDH by participating in the signaling network. GDH nevertheless appears to be associated with stress responses.

Issue date

2003-11-01

Date available

2003-11-25

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

Views

216

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