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| Identifier |
000362263 |
| Title |
Έκφραση του βακτηριακού γονιδίου hopX1 (avrPstE) σε φυτά N. benthamiana |
| Alternative Title |
Expression of bacterial gene hopX1 (avrPstE) in N. benthamiana plants |
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Author
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Παπανικολάου, Αντιγόνη
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Thesis advisor
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Πανόπουλος, Νικόλαος
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| Abstract |
Previous studies established that phypathogenic bacteria and fungi engage the mechanism of RNA silencing during their interaction with plants and this is thought to be essential for disease development in some pathosystems. Previous experiments carried out in our lab showed that certain Type III effector proteins from P.syringae pathovars act as enhancers of the RNA mediated silencing mechanism. HopX1 from Pseudomonas syringae pv. tomato DC3001 is one such effector. To further examine the involvement of RNA silencing and its consequences in the host-pathogen interaction a 35S::hopX1 expression cassette was introduced into Νicotiana benthamiana by Agrobacterium-mediated transformation of leaf discs. Transgenic plants of the Τ0/T1 generation were characterized by Northern and Southern blotting and PCR analysis. This molecular characterization confirmed the incorporation and transcriptional expression of hopX1 gene in at least three lines. Experimental infection in two genetically transformed lines showed that they were resistant to a tumor-inducing Αgrobacterium tumefaciens strain, as expected from previous findings showing that RNA-mediated silencing of T-DNA oncogenes is required for crown call tumorigenesis.The genetically transformed lines offer a useful resource for further experiments with various plant pathogens which infect N. benthamiana in order to investigate the role of RNA silencing mechanism in the pathogenicity of other pathosystems.
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| Language |
Greek |
| Issue date |
2010-11-19 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
355 |
Έκφραση του βακτηριακού γονιδίου hopX1 (avrPstE) σε φυτά N. benthamiana
