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Identifier uch.biology.msc//2006saitakis
Title Μελέτη ειδικών αλληλεπιδράσεων κυττάρου – υποστρώματος με τη χρήση βιοαισθητήρων
Creator Saitakis, Michalis
Abstract Studying interactions between receptor molecules on the cell membrane and their ligands in their native environment is a challenging task that can offer important insight into significant interactions that occur in vivo. Systems that can study such interactions would be of great use in biological and biomedical research. The aim of this work was to use biosensor systems to study an interaction between a molecule on a cell surface and its ligand immobilized on the device surfaces. The interaction pair chosen was the MHC class I molecule, HLA-A2, on the cell surface and a monoclonal antibody, specific for HLA-A2, immobilized on the surfaces of an acoustic Love-wave biosensor and an SPR-based optical sensor. The specific interaction of HLA-A2 – bearing cells with the antibody – modified surface of the acoustic Lovewave sensor resulted in signal change, which was proportional to the number of HLA – IgG pairs formed between cell membrane and sensor surface. Kinetic analysis of the real-time binding data allowed the calculation of association and dissociation rate constants and the equilibrium binding constant for both whole cell – substrate interaction and for the membrane HLA-A2 – immobilized IgG interaction. No specific response was obtained with the SPR optical biosensor. This work is the first successful attempt to study and characterise a specific cell – substrate interaction using biosensor systems.
Issue date 2006-11-0
Date available 2006-11-07
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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