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Identifier

000374590

Title

Προσδιορισμός σακχάρων στο ελληνικό μέλι με την φασματοσκοπία NMR των πυρήνων φώσφορος-31 και άνθρακας-13

Alternative Title

Determination of saccharides in greek honey employing NMR spectroscopy of the nucleus phosphorus-31 and carbon-13

Author

Καζαλάκη, Αριστέα Ευ.

Thesis advisor

Νταής, Φώτης

Abstract

The present study refers to the application of an analytical method, based on NMR spectroscopy for the quantification of saccharides in Greek honey. At first, ³¹P NMR spectroscopy was tested for the analysis of the saccharides. However, problems arrised in the interpretation of the spectra due to serious overlapping signals in the magnetic field (11.5 Τ) where the experiments took place. As an alternative solution, ¹³C NMR spectroscopy was chosen for the analysis of the saccharides. Additionally, the mutarotation of these saccharides in aqueous solution was studied. The analysis was based on spectral parameters of model compounds, determined in the present study. 1D (¹H, ¹³C), 2D (¹³C,¹H-HMQC) NMR spectroscopy and bibliographic data was utilised for the assignment of model compounds. The samples of honey analysed originated from nectar or honeydew. The botanical origins of the samples of honey in the present study were: thymus, pine, heather, fir, chestnut, flower and citrus. The present methodology was able to detect and determine quantitatively in all the samples the following saccharides: fructose, glucose, maltose, isomaltose, nigerose, turanose, maltulose, saccharose, 1-kestose, melezitose, panose, isomaltotriose, maltotriose, erlose. Finally, there was a comparison of the quantity of every saccharide in the samples of honey analysed and honey of the same types analysed in bibliography.

Language

Greek

Subject

13C/31P NMR

1D/2D NMR

Mutoration

Ελληνικό μέλι

Σάκχαρα

Ταυτομερείωση

Issue date

2012-05-25

Collection