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Χαρακτηρισμός της ευαισθησίας και ποσοτικοποίηση της Τομογραφίας Φθορισμού
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| Identifier | 000335568 | |||
| Title | Χαρακτηρισμός της ευαισθησίας και ποσοτικοποίηση της Τομογραφίας Φθορισμού | |||
| Alternative Title | Sensitivity Characterization and Quantification in Fluorescence Tomography | |||
| Author | Ψυχαράκης, Στυλιανός | |||
| Thesis advisor | Ripoll, Jorge | |||
| Abstract |
Lately, rigorous mathematical modelling of light propagation in tissue, combined with technological advancements in photon sources and detection techniques has established optical tomography as one of the main tomographic modalities applied to molecular imaging studies. Molecular imaging can be defined as the application of non-invasive techniques for the visualization of molecular events in the cellular or subcellular level, inside intact living systems. Fluorescence Molecular Tomography (FMT) incorporates the principles of Diffuse Optical Tomography (DOT) with the use of fluorescent probes as a source of contrast [1-7] In FMT the subject that carries the fluorescent probe is exposed to light from different source positions and the emitted light is captured by detectors arranged in a spatially defined order, either on reflectance or transmittance geometry. The accumulated information is then mathematically processed, resulting in a reconstructed tomographic image. The fluorescence molecular tomographer, that is presented in this thesis has already been implemented and applied to several cases of small animal imaging with satisfactory results. Specific functional characteristics or whole organs have been imaged with quite satisfactory results.. The aim of the work presented in this thesis, though, is to test the system under more controlled conditions. The experiments dealt mainly with tissue phantoms in order to establish the sensitivity and resolution of the setup, by using subjects with a priori known fluorophores’ concentration and optical properties. We have also made an approach study about different spectral data unmixing autofluorescence removal from fluorescing samples, i.e. we tried to eliminate the emitted light from the medium surrounding the fluorophores, lessening thus the contrast and the quality of the imaging results |
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| Language | English | |||
| Subject | Diffusion Equation | |||
| Fluorescence Imaging | ||||
| Fluorescence Molecular Tomography | ||||
| Issue date | 2008-12-04 | |||
| Collection | School/Department--School of Sciences and Engineering--Department of Physics--Post-graduate theses | |||
| Type of Work--Post-graduate theses | ||||
| Views | 452 | |||
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