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Home    Λειτουργική ανάλυση του γονιδίου Mox-2 στο έμβρυο όρνιθας και σύγκριση του προτύπου έκφρασής του με το ομόλογό του στο έμβρυο μυός  

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Identifier uch.biology.msc//2001rallis
Title Λειτουργική ανάλυση του γονιδίου Mox-2 στο έμβρυο όρνιθας και σύγκριση του προτύπου έκφρασής του με το ομόλογό του στο έμβρυο μυός
Creator Rallis, Charalabos D
Abstract Διατμηματικό, συνεργαζόμενα Τμήματα Βιολογίας και Ιατρικής. Mox genes constitute a subfamily of non-clustered, antennapedia-like homeobox genes that are expressed in a wide range of mesodermal tissues and organs. Mox-2 has been cloned in Human, mouse, Xenopus, rat and chick. The Mox-2 null mice carry abnormalities in the limbs. The muscle mass is reduced and specific muscles are absent. In these animals the expression of Pax-3 and Myf-5 is downregulated suggesting a role for Mox-2 in the genetic hierarchy controlling limb muscle development. We analysed the expression pattern of chick Mox-2 and we compared it with that of its homologous gene in the mouse embryo. Important differences have been revealed in developing limbs and somites. Furthermore, we have analysed the role of chick Mox-2 using the following techniques: 1. Overexpression of Mox-2 via retroviruses, 2. In ovo neural tube ablation and 3. Disruption of Mox-2 expression via RNA interference. Our results show that Mox-2 expression is not dependent on neural tube derived signals. Mox-2 overexpression upregulates Pax-3 expression in a sub-population of limb myoblasts and downregulates Myogenin. E4 embryos which are operated at the 7-12 somite stage, the tail bud is not formed and the hindlimbs are atrophic. E6 embryos in which Mox-2 is overespressed show a heart malformation and the infected limb is smaller. In chick and mouse embryos, Mox-2 seems to be involved in cell cycle and proliferation mechanisms. Moreover, in mouse Mox-2 is implicated in determination of epithelial cell type.
Issue date 2001-01-01
Date available 2000-11-07
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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