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Identifier

000374590

Title

Ανάλυση πρωτεϊνικών συνδετικών μέσων σε έργα ζωγραφικής με τη φασματοσκοπία πυρηνικού μαγνητικού συντονισμού (NMR)

Alternative Title

Analysis of proteinaceous binding media in paintings by Nuclear Magnetic Resonance Spectroscopy

Author

Σφακιανάκη, Σοφία Γεωργίου

Thesis advisor

Σπύρος, Απόστολος

Abstract

NMR spectroscopy is a powerful analytical tool for the identification and quantative analysis of organic materials in a cultural heritage context. In the present work is presented an analytical NMR protocol for the identification and quantification of egg yolk in mixtures of binding media that also contain a drying oil, namely linseed oil. The samples studied have been artificially and/or naturally aged in order to simulate the composition of organic materials in paintings. The chemical structural characterization of the aged binding media was done via advanced 1D and 2D gradient NMR spectroscopy techniques. Analysis of the 1D and 2D NMR spectra showed that egg yolk can be identified in binding media through a series of characteristic signals originating from cholesterol present in egg yolk. Cholesterol and lipid signals in the NMR spectra are used to construct characteristic molecular markers that depend on the amount of egg yolk present in the binding medium, and can be used to estimate the chemical composition of the binding medium. The developed NMR methodology was then, successfully applied to the analysis of the binding medium in the Byzantine icon “Evangelismos”, which is dated at the late 19th century.

Language

Greek

Subject

Analytical chemistry

Cultural heritage

Egg tempera

Linseed oil

Αναλυτική χημεία

Αυγοτέμπερα

Λινέλαιο

Πίνακες ζωγραφικής

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