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Identifier

000376879

Title

Μελέτη της επίδρασης των πρωτεινών θερμικού πλήγματος 70(Ηsp70) στο στρέσ

Alternative Title

The role of Hsp70 proteins during stress

Author

Γεωργοπούλου, Ανθή

Thesis advisor

Βενυχάκη, Μαρία

Abstract

Hypothalamic‐pituitary adrenal (HPA) axis is the main endocrine axis regulating the stress response including inflammation. Heat Shock protein (HSP) expression is vital to cellular and tissue protection after stress or injury. The 70‐kDa heat shock protein (HSP70) family is a group of proteins that are critical for protein assembly, folding, and transport. Furthermore, HSP70 are involved in the inflammatory stress. Based on the above, the aim of the present study was to evaluate the role of HSP70 on the hypothalamic‐pituitary adrenal (HPA) axis during stress. For our studies we used the Hsp70 deficient mice (Hsp70‐/‐) and their wildtype littermates (Hsp70+/+) which were subjected to restraint and inflammatory pain. Our results showed that Hsp70‐/‐ mice had lower levels of CRH and POMC mRNA levels compared to Hsp70+/+ mice under basal conditions and following restraint for 3 hrs. However, plasma glucocorticoid levels did not differ between the two genotypes under basal conditions and following stress. Similarly no differences were observed between the two genotypes following 15 min of restraint. Moreover, six hours after injection with CFA, pain threshold was reduced in Hsp70‐/‐ mice in contrast to wildtype mice. Corticosterone levels, at the same time point, were reduced in Hsp70 ‐/‐ mice compared to their wildtype littermates. Our results indicate a role of HSP70 on the activation of the HPA axis during stress. The mechanism of action of HSP70 on the HPA axis activity is under investigation.

Language

Greek

Subject

Biochemistry

Flammatory pain

HPA axis

Hsp70 family

Restraint experimental

Πειράματα περιορισμού

Φλεγμονώδης πόνος

Issue date

2012-12-11