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And Author="Αλέξανδρος"
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Identifier |
000341714 |
Title |
Ο ρόλος της χοληστερόλης στο διμερισμό της πρωτεϊνης NPC1L1 |
Author
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Συκαράς, Αλέξανδρος
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Thesis advisor
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Ζαννής, Βασίλειος
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Abstract |
Intestinal cholesterol absorption plays an important role in determining the levels of circulating cholesterol. Recently a new pharmacological agent, ezetimibe, was released that reduces cholesterol absorption and plasma cholesterol levels. The molecular target of ezetimibe is likely Niemann-Pick C1 Like 1 protein (NPC1L1), which is expressed predominantly in the small intestine . Inactivatιοn of NPC1L1 in mice results in a 70% reduction in the absorption of orally administered radiolabeled cholesterol, which is similar to the reduction seen in wild-type mice treated with ezetimibe. The molecular mechanism by which NPC1L1 transports cholesterol is unknown. Previously, a post-doctoral fellow in the Hobbs/Cohen laboratory observed a cholesterol-associated change in the migration of NPC1L1 to a higher apparent molecular mass on a native gel. The first aim of my project is to determine if this higher molecular weight band represents a dimer of NPC1L1. Initial experiments have been performed by expressing differentially-tagged versions of NPCC1L1 in Chinese Hamster Ovarian(CHO-K1)cells and determining if the two forms of NPC1L1 co-immunoprecipitate (Co-IP).
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Language |
English |
Subject |
Cholesterol metabolism |
Issue date |
2007-03-08 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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Notes |
Πρόγραμμα μεταπτυχιακών σπουδών "Κυτταρική και γενετική αιτιολογία, διαγνωστική και θεραπευτική των ασθενειών του ανθρώπου". |
Permanent Link |
https://elocus.lib.uoc.gr//dlib/a/8/4/metadata-dlib-6914349825891a095472b0d73b3472c6_1237545092.tkl
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Views |
178 |