E-Locus - Institutional Repository of the University of Crete - Μέτρηση του ρυθμού πρωτεϊνοσύνθεσης σε κυτταροειδικό και ιστοειδικό επίπεδο, σε ζωντανούς νηματώδεις Caenorhabditis elegans. Μέρος Β: Λειτουργικός χαρακτηρισμός ενός μέλους της οικογένειας των DEG/ENaCs, στο νηματώδη Caenorhabditis elegans

Home Μέτρηση του ρυθμού πρωτεϊνοσύνθεσης σε κυτταροειδικό και ιστοειδικό επίπεδο, σε ζωντανούς νηματώδεις Caenorhabditis elegans. Μέρος Β: Λειτουργικός χαρακτηρισμός ενός μέλους της οικογένειας των DEG/ENaCs, στο νηματώδη Caenorhabditis elegans

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Identifier

uch.biology.msc//2007kourtis

Title

Μέτρηση του ρυθμού πρωτεϊνοσύνθεσης σε κυτταροειδικό και ιστοειδικό επίπεδο, σε ζωντανούς νηματώδεις Caenorhabditis elegans. Μέρος Β: Λειτουργικός χαρακτηρισμός ενός μέλους της οικογένειας των DEG/ENaCs, στο νηματώδη Caenorhabditis elegans

Author

Κούρτης, Νίκος

Abstract

Part Α. Currently available methodologies for measuring protein synthesis rates rely on metabolic labeling by incorporation of radioactive amino acids into nascent polypeptides. These approaches are hampered by several limitations and cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. During this thesis we tried to overcome the above limitations and we established a novel method for monitoring protein synthesis in specific cells and tissues of live Caenorhabditis elegans animals. Fluorescent reporter proteins such as GFP are expressed in specific cells and tissues of interest or throughout animals using appropriate promoters. Protein synthesis rates are assessed by following fluorescence recovery after partial photobleaching of the fluorophore at targeted sites. We evaluated the method by examining protein synthesis rates in diverse cell types of the nematode. Because it is non-invasive, our approach allows monitoring of protein synthesis in single cells or tissues with intrinsically different protein synthesis rate. Part Β. Proteins of the Degenerin family form pores in cell membranes for the passage of sodium ions, in organisms as diverse as worms, flies, hydras, sea urchins and humans. Members of this family participate in various functions like, sensing of proton gradients (ASICs), mechanosensation (DEGs), activation by peptide neurotransmitters (FaNaChs) and regulation of sodium homeostasis (ENaCs). During the present thesis we attempted the characterization of one member of the Degenerin family, namely the T28B8.5. Characterization of the expression pattern showed that this gene is expressed in neurons responsible for chemosensation, as well as in neurons with unknown function. In order to proceed to the functional characterization of this gene, we tested worms mutated for the T28B8.5 gene, in behavioral assays.

Language

Greek

Issue date

2007-11-28

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

Views

339

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