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Identifier uch.biology.msc//2005kontaki
Title Μελέτη της μετατόπισης γονιδίων προς την πυρηνική περιφέρεια υπό συνθήκες ενεργοποίησης στο saccharomyces cerevisiae
Author Κοντάκη, Χαρούλα
Abstract The aim of the current thesis was to study the mechanism through which transcriptionally active genes move towards the nuclear periphery. Published recently by P. Silver [8], the interaction between highly transcribed genes (GAL genes) and a subset of nuclear pore proteins predicts that at least some genes move to the pore upon transcriptional activation. According to previously published data, the Cyc8(Ssn6)-Tup1 corepressor complex is continuously associated with target promoters under both repressive and inducing conditions. In addition Tup1 is localized at the nuclear periphery. Taking in account these observations, it was worthwhile to study the possible role of Tup1 in the movement of GAL genes upon their transcriptional induction. In order to confirm the connection of GAL genes with components of the nuclear pore upon galactose induction, many chromatin immunoprecipitation experiments were performed. However, the published result was not repeated., leading the study to a “dead end”. A way out of this was the development of a two vector system, with which the topology of the studied genes can be observed with confocal microscopy.
Language Greek
Issue date 2005-11-28
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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