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Identifier |
000404263 |
Title |
Regulation of protein synthesis in the brain |
Alternative Title |
Ρύθμιση της πρωτεϊνοσύνθεσης στον εγκέφαλο |
Author
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Αμβροσιάδης, Θεόκλητος
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Thesis advisor
|
Καραγωγέως, Δ.
Γκόγκας, Χ.
Σιδηροπούλου, Κ.
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Abstract |
mRNA translation is a fundamental cellular function that is tightly regulated by intra-cellular signaling pathways. Translation initiation is a crucial step of this process in eukaryotic cells and is, thus, under the most extensive regulation. Dysregulation of protein synthesis in the brain has been implicated in various neuropsychiatric disor-ders, among which autism spectrum disorder (ASD) is the focus of the present study. Mutations of the contactin-associated protein-like 2 (CNTNAP2) gene are associated with development of ASD and related disorders in humans and lead to ASD-like phe-notypes in mice. However, a molecular mechanism to explain these observations is lacking. Here we show that knockout of CNTNAP2 in mice leads to excessive mRNA translation, potentially mediated by the hyperactivation of the mammalian target of rapamycin complex 1 (mTORC1) that we observed. In the second part of this thesis, we make use of a previously described technique to isolate and study adult neural stem cells (NSCs) in culture.
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Language |
English |
Subject |
Autism |
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Αυτισμός |
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Νευροψυχιατρικές διαταραχές |
Issue date |
2016-12-13 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
|
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Type of Work--Post-graduate theses
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Views |
504 |