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Home    Μελέτη της χρωματινικής δομής του γενετικού τόπου MHC τάξης ΙΙ και ταυτοποίηση της γονιδιακής σήμανσής του κατά τη μίτωση  

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Identifier uch.biology.msc//2006arabatzi
Title Μελέτη της χρωματινικής δομής του γενετικού τόπου MHC τάξης ΙΙ και ταυτοποίηση της γονιδιακής σήμανσής του κατά τη μίτωση
Author Αραμπατζή, Παναγιώτα
Abstract The class II cluster is a well studied locus in terms of its transcriptional regulation and function mainly in mammalian cells. Little is known about its chromatin structure and generally about the chromatin conformation in space. In general, the chromatin structure of transcriptionally active genes has an open architecture in contrast with genes that do not expressed. The study of chromatin accessibility in the promoter of HLA-DRA gene with the restriction endonuclease hypersensitivity assay shows differential access in this locus among the different lymphatic B cell lines Raji, RJ2.2.5, SJO and SJO R. Despite this data the nucleosome mapping in this region did not show any movement of the nucleosome neither in Raji cell line nor in SJO cell line in spite of the differential chromatin accessibility. In addition, we studied the bookmarking of class II genes during the cell cycle and mainly during mitosis. We observed the presence of the enhanceosome on the promoter of the gene and also the maintenance of the histone modifications that marks transcriptionally active genes. Finally, it is shown the presence of the general transcription machine in this region which supports the theory of the existence of transcription during mitosis.
Language Greek
Issue date 2006-03-26
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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