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Home    Πολυαμίνες και μορφογενετική έκφραση εκφυτών και πρωτοπλαστών Vitis Vinifera L. (CV sultanina) και nicotiana Tabacum L. (CV xanthi)  

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Identifier uch.biology.phd//1995christaki
Title Πολυαμίνες και μορφογενετική έκφραση εκφυτών και πρωτοπλαστών Vitis Vinifera L. (CV sultanina) και nicotiana Tabacum L. (CV xanthi)
Alternative Title Polyamines and morfogenetic responce of explants and protoplasts of Vitis Vinifera l. (CV sultanina) and Nicotiana Tabacum L. (CV xanthi)
Author Χρηστάκη-Χαμψά, Μαρία Γ
Thesis advisor Ρουμπελάκη - Αγγελάκη, Καλλιόπη
Abstract The main objective of thiw research was to investigate the possible pole of polyamines in morphogenic processe of plants and particularly in plants recalcitrant in vitro regeneration. The first approach was the determination of the titers of endogenous polyamines in leaves of in vitro grapevine(Vitis Vinifera L. vs Sultanina) and tobacco (Nicotiana Tabacum L. vs Xanthi), were used. The polyamines PUT, SPD and SPM, in all the three fractions, free,soluble conjugated and insoluble conjugated, had the highest concentration in the young leaves. Furthermore, protoplasts from leaf mesophyll from the middle part of the shoot were isolated and cultured. Polyamines were determined in order to ascertain in the variation of endogenous polyamines was correlated with cell growth and division. The uptake characteristics 14C-PUT and 14C-SPD by protoplasts were studied. Both protoplast models exhibited high uptake rates of polyamines from culture medium. The distribution of labeled put after 7h of uptaked showed that it decreased in both protoplast species in soluble fraction and increased in insoluble fraction, with time. Isolatedprotoplasts from both plant species were cultured for a period of 9 days, and levels of endogenous polyamines were determined. In both species, a variation of endogenous polyamines, coinciding with cell elogation or cell division, was observed. Finally, the titers of polyamines during callogenesis in leaf explants from grapevine were determined(Abstract truncated).
Language Greek
Subject ΑΜΠΕΛΙ - VITIS VINIFERA L. (CV SULTANINA); ΑΝΑΓΕΝΝΗΣΗ ΦΥΤΟΥ; ΚΑΛΛΟΓΕΝΕΣΗ; ΚΑΠΝΟΣ NICOTIANA TABACUM L. (CV. XANTHI); ΜΟΡΦΟΓΕΝΕΣΗ ΣΕ ΦΥΤΑ; Πολυαμίνες; Πρωτοπλάστες
Issue date 1995-12-07
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
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