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Home    Μελέτη του ρόλου της λαμινίνης του κουνουπιού An. gambiae ως υποψήφιου υποδοχέα για τη μετάδοση του παρασίτου P. berghei  

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Identifier uch.biology.msc//2004vogiatzi
Title Μελέτη του ρόλου της λαμινίνης του κουνουπιού An. gambiae ως υποψήφιου υποδοχέα για τη μετάδοση του παρασίτου P. berghei
Creator Vogiatzi, Tereza-Andriani
Abstract Malaria is still one the biggest burdens of world health. It causes one million deaths and it is estimated that 300 million people are affected annually. Anopheline mosquitoes transmit the Plasmodium parasites, which cause the disease. The area affected by malaria the most is sub-Saharan Africa, where 90% of the annual cases occur. It is believed that in the future annual malaria cases will increase in sub-Saharan Africa and occurrence will expand in other parts of Africa and Europe, since traditional control methods are currently inadequate. Therefore, scientists are now turning towards new, alternative, methods of control. The life cycle of the parasite includes a number of different stages, among these, the ookinete stage. The ookinete is a motile form that invades the midgut epithelium and subsequently reaches the basal lamina. After contacting the basal lamina the ookinete stops transforms into an oocyst. During the ookinete stage the parasites undergo severe loses, which make it an ideal candidate for the application of new control methods. As a result, many studies focus on understanding of mosquitoparasite interactions, during the vector stage of the parasite cycle, at a cellular and molecular level, to ultimately use this information to produce genetically modified refractory mosquitoes. The aim of this study is: Α) Further study of the interactions between two parasitic proteins CTRP and SOAP, and the laminin γ1 subunit, in vitro, with GST pull downs. For this purpose, CTRP and SOAP are fused to a GST tag and expressed in bacterial cultures and then purified under native conditions. Next, an interaction takes place between the purified proteins and laminin, under the correct conditions. Interactions are recognized with western blot analysis. These GST pull down experiments show a strong interaction between CTRP, SOAP and mosquito laminin. Β) Observation, with confocal microscopy, of fixed midguts stained for laminin and infected with parasites, knock out for p28, SOAP, p25, and comparison with midgets infected with wild type parasites. These experiments show co-staining between the wild type parasites and laminin. The same pattern is observed for SOAP knock out parasites.
Issue date 2004-11-01
Date available 2004-12-09
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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