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Identifier

000387784

Title

Μελέτη της βιοαποικοδόμησης του τολουολίου από το βακτήριο Pseudomonas sp. phDV1

Alternative Title

Study of tuluene biodegradation by Pseudomonas sp. phDV1

Author

Κασσωτάκη, Ελένη Εμμανουήλ

Thesis advisor

Τσιώτης, Γεώργιος

Abstract

A number of strains which belong to the Pseudomonas species have been widely used to elucidate the adaptive mechanisms underlying increased tolerance against toxic concentrations of aromatic compound organic solvents in Gram-negative bacteria, using them as a sole carbon source. A strain with high phenol-removal efficiency is Pseudomonas sp. strain phDVI, which was isolated from enriched mixed culture from samples of petroleum contaminated soil in Denmark. In the present project this strain was studied, a Gram-negative bacterium which was selected for its ability to degrade aromatic compounds. As carbon sources was used toluene, different concentrations of phenol and succinic acid. Then, a study was conducted mainly for cytoplasm protein complexes (of the bacterium when grown on different carbon sources) involved in the degradation of aromatic pollutants. Additionally, study of membrane protein complexes was conducted by treating them with 5mM EDTA and 100mM Na₂CO₃ (where optimal treatment was chosen) and then solubilization was done with 0,5% n-dodecyl-b-maltoside (DDM). The cytoplasm and membrane fractions were separated via two dimensional electrophoresis Blue Native- SDS-PAGE. Finally, a comparative study and identification of the different proteins with MALDI TOF / TOF was conducted. In conclusion, cytoplasm and membrane complexes from bacteria grown on different carbon sources were identified.

Language

Greek

Subject

Biodegradation

Blue Native page

MALDI TOF/TOF

SDS-Page

Βιοαποικοδόμηση

Μη αποδιατακτική ηλεκτροφόρηση

Οργανικοί ρύποι

Τολουόλιο φαινόλη

Issue date