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Identifier uch.biology.msc//1999zagoraiou
Title Μελέτη της ενεργότητας του μεταθετού στοιχείου Minos σε κύτταρα θηλαστικών
Creator Zagoraiou, Laskaro D
Abstract Διατμηματικό, συνεργαζόμενα Τμήματα Βιολογίας και Ιατρικής. Here we report a study of the activity of Minos transposable element in the HeLa cells. Transfection experiments with two donor plasmids carrying different in size transposons (2 kb and 8.2 kb) were performed in order to determine the effect of the transposon size to the efficiency of the transposase-mediated integrations into the genome. The efficiency was found to drastically decrease when the 8.2 kb transposon was used. The ratio of transposase vs transposon that leads to the highest number of transposase-mediated integrations was also determined. Stably transformed clones were created such as remobilization of the transposon to be assayable, in order to assess the property of Minos to transpose to a new position in the genome.
Issue date 1999-12-01
Date available 2000-06-28
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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