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Home    Μελέτη μεταλλοϊόντων και μεταλλοπρωτεϊνών σε υδατικά διαλύματα με πνευματική εκνέφωση συνδυασμένη με φασματοσκοπία πλάσματος επαγόμενου από λέιζερ (LIBS) και φασματομετρία μάζας υπερηχητικού ιοντισμού (SSI-MS).  

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Identifier 000391206
Title Μελέτη μεταλλοϊόντων και μεταλλοπρωτεϊνών σε υδατικά διαλύματα με πνευματική εκνέφωση συνδυασμένη με φασματοσκοπία πλάσματος επαγόμενου από λέιζερ (LIBS) και φασματομετρία μάζας υπερηχητικού ιοντισμού (SSI-MS).
Author Μαρματάκης, Κωνσταντίνος Ε.
Thesis advisor Άγγλος, Δημήτριος
Reviewer Περγαντής, Σπύρος
Βελεγράκης, Μιχαήλ
Abstract The objective of this study is the development of a combined molecular and atomic detection system suitable for detecting metal-containing biomolecules and complexes. The use of sonic-spray ionization (SSI) mass spectrometry (MS) was investigated in combination with Laser Induced Breakdown Spectroscopy (LIBS), ultimately aiming to fully integrate them by using a single pneumatic nebulization system for sample introduction into both. The new analytical technique will be able to provide information on both elemental composition and molecular weight of the target compounds. The pneumatic nebulizer acts as the ion source in the sonic spray mass spectrometry and as the sample introduction system in LIBS. A new experimental set up was developed for performing LIBS measurements in aerosol sprays. Plasma was generated by a nanosecond Q-Switched Nd:YAG laser, which operated at its fundamental frequency, λ = 1064 nm. The radiation emitted from the plasma was collected and imaged on an optical fiber by use of a telescope system. The spectral analysis and the recording of the collected radiation were carried out on a Czerny-Turner imaging spectrograph coupled with an ICCD detector. Mass spectrometry measurements were performed on a Thermo Finnigan LCQ Advantage spectrometer, using an ion trap mass analyzer and a sonic-spray ionization (SSI) source. A number of critical measurement parameters were optimized on the LIBS system, including laser focusing with respect to the spray cone, laser pulse energy and detection temporal window as well as light collection geometry. Under these conditions aerosols containing different metal ions such as Na+, Cu2+, Fe3+, Ca2+ και Ba2+ were analyzed by the calibration curve method. The limit-of-detection (LOD) values were in the range of 1x10-7 M to 1x10-4 M with the precision of the method being excellent, 90-99 %. Finally, the capability of probing biomolecules in aerosols with LIBS and SSI-MS separately with LIBS and SSI-MS was demonstrated by studying aqueous solutions of two metal-proteins, Superoxide-dismutase (SOD) and α-lactalbumin (LALBA).
Language Greek
Issue date 2015-03-20
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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