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Home    Μελέτη της οικογένειας των αννεξινών του Ανωφελούς κώνωπα, πρωτεϊνών που προσδένονται στο πρωτόζωο Plasmodium berghei  

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Identifier uch.biology.phd//2004kotsifakis
Title Μελέτη της οικογένειας των αννεξινών του Ανωφελούς κώνωπα, πρωτεϊνών που προσδένονται στο πρωτόζωο Plasmodium berghei
Alternative Title A study of the Anopheles annexin family as proteins that bind Plasmodium berghei parasites
Author Κοτσυφάκης, Μιχάλης
Abstract The aim of this study is to uncover polypeptides in the mosquito Anopheles gambiae that strongly interact with the malaria parasite Plasmodium berghei and study their putative functional role during the infection of the mosquito midgut. Distinct molecules (with an apparent molecular weight between 34 kDa and 52 kDa) were detected to selectively bind purified ookinetes in vitro, not only in An. gambiae protein extracts, but also in extracts from other dipteran insects, including the mosquito Aedes aegypti and the fruitfly Drosophila melanogaster. Combining two-dimensional electrophoresis and MALDI-TOF mass spectrometry, the fruitfly polypeptides were identified as isomorphs of the annexin B11 protein. The Anopheles orthologues were subsequently cloned and their expression in the mosquito tissues was studied. Immunostaining of midguts after ingestion of a Plasmodium-infected blood meal, showed that annexin binding can be detected in vivo during the onset of malaria infection. Functional analysis using RNAi techniques and mosquito feeding with anti-annexin antibodies, indicates that annexin-Plasmodium interaction is advantageous for the developing malaria parasites, thus promoting the infection of the Anopheles midgut. The results of this work could contribute in the development of novel strategies to fight the disease, that target Plasmodium development in the invertebrate vector.
Language Greek
Subject Ελονοσία
Μεσέντερο
Issue date 2004-11-23
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
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