E-Locus - Institutional Repository of the University of Crete - Μελέτη των επιπέδων έκθεσης σε ενδοκρινικούς διαταράκτες της πληθυσμιακής ομάδας (κοόρτης) μητέρας-παιδιού "ΡΕΑ" (Κρήτη)

Home Μελέτη των επιπέδων έκθεσης σε ενδοκρινικούς διαταράκτες της πληθυσμιακής ομάδας (κοόρτης) μητέρας-παιδιού "ΡΕΑ" (Κρήτη)

Results - Details

[Add to Basket]

Identifier

000391237

Title

Μελέτη των επιπέδων έκθεσης σε ενδοκρινικούς διαταράκτες της πληθυσμιακής ομάδας (κοόρτης) μητέρας-παιδιού "ΡΕΑ" (Κρήτη)

Alternative Title

Evaluation of exposure to endocrine disruptors among mother-child pairs in Greece (RHEA COHORT)

Author

Μυριδάκης, Αντώνης Ιω

Thesis advisor

Στεφάνου, Ευριπίδης

Reviewer

Βασιλικογιαννάκης, Γεώργιος
Θερμού, Κυριακή
Ορφανόπουλος, Μιχαήλ
Περγαντής, Σπυρίδων
Σπύρος, Απόστολος
Χανιωτάκης, Νικόλαος

Abstract

Phthalate esters (PEs), bisphenol-A (BPA) and parabens (PBs) are used widely in everyday products. They have endocrine disrupting properties and are linked with adverse health effects. Humans exposed to these chemicals, metabolize and excrete them mostly via urine. Urinary metabolite concentrations are used as biomarkers of exposure. A liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed in order to determine eight PEs metabolites, six PBs and BPA in human urine. For the first time: I) all three categories of the above endocrine disruptors were simultaneously extracted from human urine and II) the separation of two pairs of structural isomers, namely, iso-/n- butyl paraben and propyl paraben was achieved with a conventional reversed phase LC column. The developed method was applied to samples from two hundred and thirty nine (239) pregnant women, their 2.5-year old children (239), and five hundred (500) 4-year old children, all subjects of the “Rhea” cohort (Crete). Concentration levels of BPA and PEs metabolites were comparable with other studies worldwide. PBs levels were higher compared to a relevant study conducted in Denmark. We observed for all examined compounds, except for di-2-ethylhexyl phthalate (DEHP) and BPA, higher median daily intake (DIu) for mothers than for their children. DIu for 4-year children was lowest compared to the corresponding for 2.5- year old children. Low correlations (two-tailed spearman; CC 0.1-0.2; p< 0.01) were observed for di-ethyl phthalate (DEP), di-n-butyl phthalate (DnBP), butyl-benzyl phthalate (BBP) and ethyl paraben (EPB) concentrations between mothers and their children. Exposure was assigned to two main sources: a) plastics related to PE-BPA and, b) to personal care-hygiene products related to PB and DEP (di-ethyl phthalate). DEHP metabolism seems to be related to age and sex. Indoor air and drinking water PEs daily intake represented a small fraction of the total daily intake.

Language

Greek

Subject

Bisphenol-A

Electrospray ionisation

Exposure biomarkers

Liquid chromatography

Motherchild cohort Rhea

Parabens

Phthalate esters

Preschool-age children

Tandem mass spectrometry

Βιοδείκτες έκθεσης

Δίδυμη φασματομετρία μάζας

Δισφαινόλη-Α

Ενδοκρινικοί διαταράκτες

Ηλεκτροψεκασμός

Κοόρτη μητέρας-παιδιού «ΡΕΑ»

Παραβένια

Υγρή χρωματογραφία

Φθαλικοί εστέρες

Issue date

2015-03-18

Collection

School/Department--School of Sciences and Engineering--Department of Chemistry--Doctoral theses

Type of Work--Doctoral theses

Views

575

Digital Documents
	Download document View document Views : 4
	Download document View document Views : 15