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Title

Σύνθεση βιοϋβριδίων πρωτεΐνης-πολυμερούς μέσω εκλεκτικής αζω-βιοσύζευξης σε ομάδες πρωτεϊνικής τυροσίνης

Alternative Title

Synthesis of protein-polymers biohybrids via selective tyrosine azo-bioconjugation

Author

Μανάι, Γιούντι

Thesis advisor

Βελώνια, Καλλιόπη

Abstract

The purpose of this thesis was to synthesize amphiphilic protein-polymer conjugates, a class of synthetic biomaterials that have potential applications in biomedicine, nanotechnology, biotechnology and photonics. To achieve this, we syntesized a protein biomacroinitiator via covalent conjugation of an azo-functionalized-radical initiator to tyrosine residues of proteins such as bovine serum albumin (BSA) or lysozyme. A grafting from, oxygen tolerant RDRP approach, was applied to grow polymers from the biomacroinitiator following either a photoinduced, Cu(II) mediated, or a SET-LRP, Cu(I) mediated approach. The bioconjugates were characterized with gel electrophoresis (PAGE), Size Exclusion Chromatography (SEC) and UV-Vis spectrophotometric studies.

Language

Greek

Issue date

2021-03-26

Collection

School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses

Type of Work--Graduate theses

Views

328

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