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| Identifier |
uch.biology.phd//2003topalidou |
| Title |
Στρατολόγηση και λειτουργία μεταγραφικών πολυπρωτεϊνικών συμπλόκων in vivo |
| Alternative Title |
Recruitment and function of transcriptional protein complexes |
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Author
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Τοπαλίδου, Ειρήνη
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Author
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Δελιδάκης, Χρήστος
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| Abstract |
Regulation of gene expression opperates mainly at the level of transcription and results as a consequence of the co-ordinated activity of two categories of regulatory elements, promoters and activators. In this study the in vivo role of both these elements in the recruitment and function of transcriptional complexes was examined. Firstly, the contribution of the promoter architecture in such phenomena was studied. It was revealed that the quality of the TATA element plays a role in nucleosome modifications and selection of coactivators. More importantly this study revealed the existence of a co-transcriptional mechanism that determines the levels of Gcn5-dependent histone acetylation within promoter nucleosomes. Secondly, studies on the contribution of the activator for the recruitment and function of transcriptional complexes revealed a role of the activation domains in determining the transcriptional requirement for specific SAGA components. In addition, a novel function of the Spt3 component of SAGA and of the Mot1 ATPase in nucleosome remodeling was demonstrated. In the last part the study of the contribution of cis-elements revealed the ability of activators to associate in vivo with non promoter euhromatic sites. This association was shown to result in the functional recruitement of SAGA and Swi2/Snf2 complexes. This assemblage of coactivator complexes was proven not to be productive because of the absence of core promoter elements other than the TATA box that are required for stable recruitement of the Mediator.
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| Language |
Greek |
| Subject |
Μεταγραφικές ρυθμίσεις; Ενεργοποιητής; Υποκινητής; Μεταγραφικά σύμπλοκα; Νουκλεοσωμική αναδιαμόρφωση; Ακετυλίωση; Στρατολόγηση παραγόντων; Χρωματίνη |
| Issue date |
2003-11-26 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
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Type of Work--Doctoral theses
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| Views |
271 |
Στρατολόγηση και λειτουργία μεταγραφικών πολυπρωτεϊνικών συμπλόκων in vivo
