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Identifier

000419743

Title

Study of exosomal pathways in Hodgkin and non-Hodgkin lymphoma cells after p53 re-activation

Alternative Title

Μελέτη των εξωσωμικών μονοπατιών σε Hodgkin και non-Hodgkin κύτταρα λεμφώματος μετά από επαν-ενεργοποίηση της p53.

Author

Καλαντίδου, Αικατερίνη Π.

Thesis advisor

Αϊβαλιώτης, Μιχαήλ

Reviewer

Αθανασάκη, Ειρήνη
Κοκκινίδης, Μιχαήλ

Abstract

The role of exosomal pathways is important for the maintenance of cellular homeostasis, especially in tumorigenesis. Thus, the deciphering of the proteome of lymphoma wild type p53 would help us understand the patho-biology of lymphoma. In this study, our purpose was to identify the differentially expressed proteins which are associated with exosomes, the evaluation of their interaction and their therapeutic role after p53 re-activation with Nutlin (N3a) in three different type of lymphoma. In vitro human lymphoma cell line model systems -/+ N3a were subjected to comparative proteomic and advanced bioinformatics analysis. In total, 4037 proteins were identified and 77 proteins of them were associated with exosomes. Moreover, the analysis of the protein interaction network revealed their functional association and interaction between the proteins, indicating possible markers for targeting in human lymphomas. In conclusion, our study aims to investigate the role of biogenesis and secretion of exosomes in lymphomas and the tolerance of lymphoma in therapeutic approaches after p53 activation.

Language

English

Subject

Nutlin3a

Proteonics

Wild type p53

Άγριου τύπου p53

Εξωσωμικά μονοπάτια

Λέμφωμα

Issue date

2018-11-23

Collection