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Identifier 000412896
Title Παραγωγή και χαρακτηρισμός βιοεμπνευσμένων υλικών σε βακτήρια
Alternative Title Production and characterisation of bioinspired materials in bacteria
Author Πριγκηπάκη, Αριάδνη Ι.
Thesis advisor Κοκκινίδης, Μιχάλης
Reviewer Μητράκη, Α
Πετράτος, Κ.
Φαρσάρη, Μ.
Ρανέλλα, Α.
Χατζηνικολαΐδου, Μ.
Χαλεπάκης, Γ.
Abstract Fibrous proteins are the building blocks of many biological tissues and natural materials such as wool and silk. They often display very interesting mechanical and elastic properties and serve as structural scaffolds for the design and production of artificial scaffolds as they provide support for cell attachment, proliferation and differentiation. A class of fibrous proteins is used by viruses and bacteriophages as adhesins. In previous studies octapeptides derived from the adenovirus fiber shaft sequence that self assemble into fibrils, were used to decorate 3D structures for the deposition of calcium minerals. The subject of the present thesis is a protein that corresponds to the adenovirus fiber shaft fragment and has the propensity to self assemble into fibrils. The first part is focused on the expression and structural characterization of the protein corresponds to amino acids 61 – 392, while in the second part a laser processing technique is presented using single nanosecond pulses of a KrF laser (λ=248 nm) for the treatment of protein films cast on quartz plates. After laser treatment of the film surface, a new foamy layer was formed upon single pulse irradiation. This laser induced micro foam structure was characterized by several microscopic methods, whereas the photochemical modifications by employing the laser induced fluorescence (LIF) technique in order to detect the differences between the irradiated surfaces compared to the non-irradiated ones. Finally the attachment and proliferation of a model fibroblast cell line on the irradiated and non-irradiated protein films, was tested and compared. The results indicated that the cells attach selectively to the irradiated areas of the protein film, therefore the laser processing of protein films that we proposed, can be used as a method for inducing selective cell patterning at the microscale as it is a rapid and low cost operation.
Language Greek
Subject Cell lines
KrF
Laser
Scaffolds
Ακτινοβόληση
Ικριώματα
Κυτταροκαλλιέργεια
Issue date 2018-02-08
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
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