|
Identifier |
000412896 |
Title |
Παραγωγή και χαρακτηρισμός βιοεμπνευσμένων υλικών σε βακτήρια |
Alternative Title |
Production and characterisation of bioinspired materials in bacteria |
Author
|
Πριγκηπάκη, Αριάδνη Ι.
|
Thesis advisor
|
Κοκκινίδης, Μιχάλης
|
Reviewer
|
Μητράκη, Α
Πετράτος, Κ.
Φαρσάρη, Μ.
Ρανέλλα, Α.
Χατζηνικολαΐδου, Μ.
Χαλεπάκης, Γ.
|
Abstract |
Fibrous proteins are the building blocks of many biological tissues and natural materials
such as wool and silk. They often display very interesting mechanical and elastic properties
and serve as structural scaffolds for the design and production of artificial scaffolds as they
provide support for cell attachment, proliferation and differentiation. A class of fibrous
proteins is used by viruses and bacteriophages as adhesins. In previous studies octapeptides
derived from the adenovirus fiber shaft sequence that self assemble into fibrils, were used
to decorate 3D structures for the deposition of calcium minerals.
The subject of the present thesis is a protein that corresponds to the adenovirus fiber shaft
fragment and has the propensity to self assemble into fibrils. The first part is focused on
the expression and structural characterization of the protein corresponds to amino acids 61
– 392, while in the second part a laser processing technique is presented using single
nanosecond pulses of a KrF laser (λ=248 nm) for the treatment of protein films cast on
quartz plates. After laser treatment of the film surface, a new foamy layer was formed upon
single pulse irradiation. This laser induced micro foam structure was characterized by
several microscopic methods, whereas the photochemical modifications by employing the
laser induced fluorescence (LIF) technique in order to detect the differences between the
irradiated surfaces compared to the non-irradiated ones. Finally the attachment and
proliferation of a model fibroblast cell line on the irradiated and non-irradiated protein
films, was tested and
compared.
The results indicated that the cells attach selectively to the irradiated areas of the protein
film, therefore the laser processing of protein films that we proposed, can be used as a
method for inducing selective cell patterning at the microscale as it is a rapid and low cost
operation.
|
Language |
Greek |
Subject |
Cell lines |
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KrF |
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Laser |
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Scaffolds |
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Ακτινοβόληση |
|
Ικριώματα |
|
Κυτταροκαλλιέργεια |
Issue date |
2018-02-08 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
|
|
Type of Work--Doctoral theses
|
Views |
311 |