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Identifier

000369232

Title

Ταυτοποίηση ανοσορυθμιστικών πρωτεϊνών από το φυτό Phlomis lanata

Alternative Title

Identification of immuno-regulatory proteins from the plant Phlomis lanata

Author

Καράλη, Αικατερίνη-Δεβόρα

Thesis advisor

ΑΘανασάκη, Ειρήνη

Abstract

Plants and their derivatives have been used through centuries in therapeutics and wound healing. Plant essential oils from the genus Phlomis have been widely studied for their anti-microbial and anti-oxidant properties. Phlomis lanata belongs to this genus and it is considered to be one of Crete’s endemic plants. To date, there are studies on the properties of the essential oils and extracts of this plant but no research in regard to its proteins. In the present study, we investigated the possible effect of the proteins of Phlomis lanata on the immune system of mice. Upon fractionation, protein extracts were applied to total spleen cells and their effect on the proliferation of the these cells as well as the cytokine production was examined. To have a more thorough investigation, the T cell sub-populations were isolated, based on the presence or absence of the CD25 and CD4 receptors. Among the 13 fractions tested, two protein fractions, namely W1 and 600mM NaCl, were shown to suppress and stimulate spleen cell proliferation, respectively. Taking into account the cytokine profile as well as the effect of these fractions on the isolated T cells, it was shown that the suppressive activity of the fraction W1 was mainly due to IL-10 and TNFa increase, while the stimulatory activity of 600mM NaCl was due to the inhibition of T regulatory cells. These results open new areas of research as to the regulatory role of P. lanata protein extracts on the immune system.

Language

Greek

Subject

CD25

CD4

IL-10

IL-2

T-cells

TNFa

Issue date

2011-11-18

Collection