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| Identifier |
000444626 |
| Title |
Chapter I: Investigating potential pathways that the newly discovered SFH8 protein may participate in. Chapter II: The role of Processing Bodies (PBs) in protein trafficking in A. thaliana L. |
| Alternative Title |
Κεφάλαιο Ι: Διερεύνηση πιθανών μονοπατιών στα οποία μπορεί να συμμετέχει η πρόσφατα ανακαλυφθείσα πρωτεΐνη SFH8. Κεφάλαιο ΙΙ: Ο ρόλος των Processing Bodies (PBs) στη μεταφορά πρωτεϊνών στο φυτό Arabidopsis thaliana L. |
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Author
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Παρασκευοπούλου, Δάφνη Ι.
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Thesis advisor
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Μόσμου, Παναγιώτης
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| Abstract |
Chapter I:The Sec14p-nodulin family is a Phosphatidylinositol (PtdIns) transfer protein (PITPs)
subfamily, conserved among species. The current knowledge about the functions and roles of the
proteins in this family, in plants, is still poor and vague. However, a new SEC14-like protein (SFH8)
discovered in Arabidopsis thaliana L. provides insight in polarity establishment mechanism, unveiling
several new properties and functions of these proteins. In order to expand our knowledge on the
pathways in which this protein is implicated and identify other proteins which SFH8 interacts with,
we applied a forward genetic approach, the EMS mutagenesis, on the already mutated sfh8-1 plants.
Through screening of more than 100 families of plants in the M2 generation, we aimed to find a
phenotype that could ‘rescue’ the mutant plant. In results, there were some individuals that showed
epistasis on the mutant gene. This could open a new series of experiments that may further clarify
SFH8 roles in plant growth.
Chapter II: Due to their sessile nature and their need to rapidly response to environmental challenges,
plants have developed a conserved mechanism to control their gene expression. The processing
bodies (PBs) are membraneless RNA granules formed via liquid–liquid phase separation (LLPS)
and are important for mRNA decay and temporal storage of translationally silenced mRNAs. In
plants, PBs play significant roles in plant development and response to stress. Recent analysis of
the PB interactome revealed that DCP1, a major PB component, forms interactions with proteins
involved in the endocytic cycle and membrane protein remodeling. In this study, we aimed to
elucidate the relationship of the Endosomal Sorting Complex Required for Transport (ESCRT)-
mediated protein uptake and degradation pathway with the PB components and the possible roles
of PBs in the endoplasmic reticulum – plasma membrane (ER-PM) contact sites, through the
interaction of DCP1 with VAP27-1, a conserved ER membrane protein. Results showed that ESCRTI complex seems to implicate more with PBs and that DCP1 protein might have certain roles on the
plasma membrane, also according to its phosphorylation status.
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| Language |
English |
| Subject |
DCP1 |
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ESCRT |
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Endocytosis |
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Endoplasmic reticulum – plasma membrane contact sites |
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Membrane protein trafficking |
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Ενδοκύτωση |
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Μεταφορά μεμβρανικών πρωτεϊνών |
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Σημεία επαφών ενδοπλασματικού δικτύου-Πλασματικής μεμβράνης |
| Issue date |
2022-03-28 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
300 |
Chapter I: Investigating potential pathways that the newly discovered SFH8 protein may participate in. Chapter II: The role of Processing Bodies (PBs) in protein trafficking in A. thaliana L.
