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| Identifier |
uch.med.phd//1994makrigiannakis |
| Title |
In vitro μελέτη της έκφρασης και της ρύθμισης της έκλυσης των οπιοειδών πεπτιδίων στα επιθηλιακά κύτταρα του ανθρώπινου ενδομητρίου |
| Alternative Title |
In vitro study of expression and regulation of endometrial opioids secretion in Human |
| Creator |
Makrigiannakis, Antonis S
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| Abstract |
The endogenouw opioid peptides (EDP)β-endorphin and dynorphin derive from two different families of EOP precursors products of two different genes. EOP are produced not only in the central nervous system but in non-neural tissues where, as it appers, they act locally via paracrine mechanisms.These opioids have been shown to be produced at multiple sites along the mammalian reproductive tract including the rodent uterus. The aim of the present work was to find out if the human endometrial cells express these two EOP. As an vitro model we used the Ishikawa endometrial cells, which have been shown to be a good model for the study of the effects of steriod hormones on human epithelial endometrium. Northern blot hybridization of Ishikawa cells RNA showed the presence of a 1.2 Kbases POMC and a 2.4 Kbases PDYN trancripts. Radioimmunoassay and gel filtration chromatography characterization of the immunoreactive (IR)opioid peptides in the culture media showed the presence of IR-β-endorphin and IR-dynorphins. The apparent molecular weight of IR-β-endorphin was thatof authentic β-endorphin while the bulk of the IR-dynorphin was 8 Kdaltons. The secretionof both opioids could be increased by KCL-induced depolarization. Estrogen and glucocorticoids decreased, in a dose depended manner, the secretion of β-endorphin from the Ishikawa cells while progesterone and dihydrotestosterone did not have a statistically significant effect. The antiprogestin-antiglucocorticoid RU 486 acted as an agonist i.e. it diminished β-endorphin secretion possibly via glucocorticoid receptors.
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| Issue date |
1994-01-01 |
| Date available |
1997-06-6 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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| Views |
203 |
In vitro μελέτη της έκφρασης και της ρύθμισης της έκλυσης των οπιοειδών πεπτιδίων στα επιθηλιακά κύτταρα του ανθρώπινου ενδομητρίου
