E-Locus - Institutional Repository of the University of Crete - Συν-ρύθμιση των IFNG και IFNGR1 στο γενωμικό επίπεδο

Home Συν-ρύθμιση των IFNG και IFNGR1 στο γενωμικό επίπεδο

Results - Details

[Add to Basket]

Identifier

000382143

Title

Συν-ρύθμιση των IFNG και IFNGR1 στο γενωμικό επίπεδο

Alternative Title

Co-regulation of IFNG and IFNGR1 at the genomic level

Author

Δεληγιάννη, Χρυσούλα.

Thesis advisor

Σπηλιανάκης, Χαράλαμπος
Τσατσάνης, Χρήστος

Abstract

The development of the immune system and the host immune response to microbial infections rely on the transcriptional activation and silencing of multiple gene loci. By utilizing the differentiation system of naive CD4⁺, TH1 and TH2 lymphocytes, we focused on the transcriptional regulation of the interferon-γ (Ifnγ, mouse choromosome 10) and interferon-γ receptor 1 (IfnγR1, mouse chromosome 10) genes. So, in the present doctoral thesis we present a model whereby there are long range monoallelic intra-chromosomal associations between the IfnγR1 and Ifnγ loci, in cell types that express the receptor. Expression profile analysis revealed the functional significance of the co-localization, since IfnγR1 is expressed in naive CD4⁺ and TH1 cells from the allele that is in close physical proximity with the Ifnγ locus. Moreover, the interactions take place in transcription factories, but they are independent of transcription. Importantly, CTCF, a general transcription factor, seems to play a critical role to the aforementioned phenomena. Regarding the functional characterization of the underlying mechanism, here we provide evidence to support the hypothesis that CTCF is recruited to the non-methylated promoter region of IfnγR1 allele, possibly with the help of Τ-bet and sets a pattern of monoallelic co-localization and positive transcriptional regulation of the receptor gene locus.

Language

Greek

Issue date

2013-07-16

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses

Views

223

Digital Documents
	Download document View document Views : 8