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| Identifier |
000355436 |
| Title |
Μελέτη μοριακών μηχανισμών παθογένειας του φορέως πυρετού Q coxiella burnetii ενός ενδοκυτταρικού βακτηρίου |
| Alternative Title |
Study of molecular mechanisms of the pathogenesis of the Q fever agent coxiella burnetii, an intracellular bacterium |
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Author
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Παπαδιώτη, Αναστασία Αθανασίου
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Thesis advisor
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Τσιώτης, Γεώργιος
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| Abstract |
The etiological agent of Q fever is Coxiella burnetii, an obligatory intracellular
Gram-negative bacterium, the only known to date that survives and replicates
within the eukaryotic phagolysosome. Human Q fever exhibits a wide range of
clinical manifestations and the reason for this polymorphism has not been
clarified so far. Predisposing host factors are critical to the clinical outcome of
the disease, but it is quite possible that unique bacterial factors are also
important. The latter is supported by the genetic diversity which is displayed
between Coxiella burnetii strains (Bear et al, 2009, Voth et al, 2009) and a
distinction in strain virulence in models of acute Q fever (Russell-Lodrigue et
al, 2009). However, proteins are the final operators of every cellular process
and there is a lack of studies of C. burnetii strains in the protein level.
The questions that were set in this project are the followings: 1) How does
protein composition differentiate between different C. burnetii strains? 2) How
does the host cell respond to infection with different C. burnetii strains?
To answer these questions, two C. burnetii strains which have been isolated
from different sources and are associated with different clinical forms of Q
fever, were used and studied by the proteomic approach. Data analysis
revealed important virulence factors that were differentiated between the two
strains, suggesting a more or less aggressive biological behavior for each
strain. Moreover the two C. burnetii strains displayed a different profile of
intracellular growth and parasitism, underlying their different source of origin.
Q fever diagnosis is mainly based on serological tests. Whole cell bacterium
C. burnetii is used as antigen in these tests resulting in lower specificity.
Consequently there is a need for more specific antigens, thus the next goal of
this project was the development of a protocol for the detection of new
serodiagnostic markers of Q fever. This goal was achieved using an
immunoproteomic approach.
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| Language |
Greek |
| Subject |
COFRADIC |
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Coxiella burnetii |
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Coxiella burnetii strains |
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antigenic proteins |
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differential protein analysis |
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gel-based proteomics |
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gel-free proteomics |
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immunoproteomics |
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αντιγονικές πρωτεΐνες |
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διαφορική ανάλυση έκφρασης πρωτεϊνών |
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πρωτεομική |
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στελέχη C. burnetii |
| Issue date |
2010-02-02 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Doctoral theses
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Type of Work--Doctoral theses
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| Views |
304 |
Μελέτη μοριακών μηχανισμών παθογένειας του φορέως πυρετού Q coxiella burnetii ενός ενδοκυτταρικού βακτηρίου
