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Identifier uch.biology.phd//2002klinakis
Title Το μεταθετό στοιχείο Minos:ενεργότητα και εφαρμογές σε έντομα και θηλαστικά
Alternative Title The transposable element Minos: activity and applications in insects and mammals
Author Κλινάκης, Απόστολος
Thesis advisor Δελιδάκης, Χρήστος
Abstract The transposable element Minos, originally identified in Drosophila hydei, belongs to the mariner/Tc1 superfamily of transposable elements, members of which, some active, some not, have been found in retain the ability to transpose, even in hosts evolutionary distant from the elements original host, with the only prerequisite being the presence of active transposase, the enzyme that catalyzes the transposition. The present study shows that Minos, being a Dipteran element, is capable of precise excision and transposition fom one extrachromosomal site to another, in cell lines of another Dipteran, mosquito Aedes aegypti, as well as of the Lepidopteran Spodoptera frugiperda. The excision and trasposition assays. The advantage of these assays lies in the fact that they can be applied easily and fast in any host, provided a cell line of it is available in order to investigate the activity of Minos in its cells. As show in the present study, the Minos transposable element is also able to transpose in the genome and stably transform human cells. The precision and efficiency of transposition in human cells confirms the hypothesis that transposition of the members of the superfamily is not subject to limitations other than the presence of their transposase. Taking advantage of the ability of Minos to carry exogenous DNA, we devised vectors which allow the trapping of constitutively active genes in human cells. The efficiency of transposition and the number of insertions were sufficient to allow tagging all expressed genes in human cells and thus proves Minos to be a useful tool for functional genomics in mammals as well as in insects.
Language Greek
Subject Έντομα; Θηλαστικά; Παγίδευση γονιδίου; Σήμανση γονιδίου; Στοιχείο Minos; Κύτταρα
Issue date 2002-04-17
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
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