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Identifier

000369247

Title

Μελέτη της έκφρασης των τάξης ΙΙ μορίων του MHC στη μικρογλοία

Alternative Title

Regulation of MHC II expression in microglia

Author

Καλογνώμου, Μαρία

Thesis advisor

ΑΘανασάκη, Ειρήνη

Abstract

In the last decade, microglia activation has been the subject of intense investigation since it can promote a variety of events ranging from neuroprotection to neurodegeneration. Moreover, in neurodegenerative conditions like ageing, Alzheimer’s disease, Parkinson’s disease, Huntington’s disease and schizophrenia, studies have shown that deregulation of microglial activation mechanisms plays a critical role in the development of pathological conditions related to these diseases. The neurodegenerative role of microglia has been highly correlated with the expression of MHC-II molecules. The aim of the present study was to define the surface and intracellular expression of H-2A and also the intracellular expression of H-2M, H-2O and CD74 in different activation states of microglial cells, using the BV-2 cell line. Flow Cytometry, RT-PCR and Confocal microscopy analysis show that microglial cells express all necessary components for post-translational regulation of MHC class II molecules and their transport to the cell surface. Additionally, stimulation of BV-2 cells with LPS, IFN-γ and IL-4 demonstrated that different activation stimuli can lead to different activation pathways of microglia. Studying the regulatory pathways of microglial MHC II expression could delineate mechanisms of stimulation versus suppression, surface expression versus secretion of MHC-II molecules during pathological conditions in neurodegenerative diseases and dictate new strategies of therapeutic approaches.

Language

Greek

Subject

Antigen presentation

BV-2 cell line

Microglial activation

Αντιγονοπαρουσίαση

Ενεργοποίηση Μικρογλοίας

Κυτταρική σειρά BV-2

Issue date

2011-11-18

Collection