|
|
| Identifier |
000367185 |
| Title |
Ο ρόλος των ερπητοϊών στην ανδρική υπογονιμότητα |
| Alternative Title |
The implication of herpes viruses in male infertility |
|
Author
|
Νεοφύτου, Ειρήνη Ν
|
|
Thesis advisor
|
Μακρυγιαννάκης, Αντώνιος
|
|
Reviewer
|
Σπαντίδος, Δημήτριος
Σουρβίνος, Γεώργιος
|
| Abstract |
Infertility accounts for one of the problems the affect almost
15-20% of the couples in European countries. 30% of the
patients suffer from idiopathic infertility. Raising evidence
suggest that viral infections contribute to male infertility. Viral
DNA from the members of the Herpesviridae family (HSV-1,
HSV-2, CMV or EBV), have been detected in the semen of
asymptomatic sterile patients.
The aim of this PhD thesis was the detection of the 8
members of the Herpesviridae family in semen samples and
the correlation of their presence or no with the samples’
altered semen parameters.
Firstly, fresh semen samples were collected and
characterized. Secondly, the detection experiments for the
herpes viruses were performed by PCR. Thirdly, statistical
analysis was performed. In vivo experiments were also
conducted in order to confirm the capacity of infection of
CMV in spermatozoa. Semen samples were incubated in
culture medium with the virus.
The semen showed high viral presence (92,8%)
independently of their characterization.
Interestingly, there was a statistical significance between
the EBV presence and the pyospermia. The presence of EBV
influences the concentration of WBCs detected in semen.
The HHV-6 presence was statistically significant. These
results suggest that HHV-6 affects those semen parameters
concerning seminal fluid.
The high presence of HHV-8 was statistically significant
in samples with oligoasthenozoospermia. A statistical
significant correlation was also found between samples
positive for HHV-8 and reduced concentration of spermatozoa
(p=0,030) and reduced motility (p=0,015). Concluding, the
presence of HHV-8 in sperm samples seems to cause
oligoasthenozoospermia.
The results of this PhD Thesis suggest the implication of
herpes viruses in altered semen parameters, while their
detection could be incorporated in the routine semen analysis
together with the spermogram and applied both to patients
seeking fertility evaluation and those performing IVF.
|
| Language |
Greek |
| Subject |
Cytomegalovirus (CMV) |
|
Herpes simplex virus (HSV) |
|
Herpes viruses |
|
Herpesviridae |
|
Herpesviridae Infections |
|
Kaposi sarcoma associated herpesvirus (HHV-8) |
|
Male infertility |
|
Oligoasthenozoospermia |
|
Polymerase chain reaction (PCR) |
|
Sperm culture |
|
Αλυσιδωτή αντίδραση πολυμεράσης |
|
Ανδρική υπογονιμότητα |
|
Απλός έρπητας |
|
Ερπητοϊοί |
|
Ιός σχετιζόμενος με σάρκωμα καποσι |
|
Καλλιέργεια σπερματοζωαρίων |
|
Κυτταρομεγαλοϊός |
|
Ολιγοασθενοζωοσπερμία |
| Issue date |
2010-07-20 |
| Collection
|
School/Department--School of Medicine--Department of Medicine--Doctoral theses
|
|
|
Type of Work--Doctoral theses
|
| Views |
299 |
Ο ρόλος των ερπητοϊών στην ανδρική υπογονιμότητα
