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| Identifier |
000454463 |
| Title |
Ο ρόλος του MIR-144 στον πολλαπλασιασμό και τη μετανάστευση μη μολυσμένων και μολυσμένων με τον ανθρώπινο κυτταρομεγαλοϊο (HCMV) κυττάρων γλοιοβλαστώματος |
| Alternative Title |
The role of MIR-144 in the proliferation and migration of human cytomegalovirus (HCMV) infected and non-infected glioblastoma cells |
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Author
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Θωμαϊδου, Αρσινόη Χρυσοβαλάντη
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Thesis advisor
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Σουρβίνος, Γεώργιος
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Reviewer
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Ζουμπουρλής, Βασίλειος
Αγγελάκη, Σοφία
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| Abstract |
Glioblastoma (GBM) is one of the most common and deadly malignant brain tumors. Due to its highly aggressive nature, it remains an incurable disease with very poor prognosis and a median survival rate of less than 14 months after diagnosis. It has been reported that human cytomegalovirus (HCMV) and glioblastoma are often closely associated, as HCMV DNA and viral gene products have been discovered in the majority of tumor tissue samples of GBM patients. HCMV appears to play an oncomodulary role in glioblastoma, promoting the malignant properties of cells through the regulation of several cellular pathways involved in cancer development and progression. Micro-RNAs are important regulators of the oncogenetic process, either suppressing or promoting the malignant properties of cancer cells depending on their target-genes. miR-144 is a member of the cluster miR-144/451 and has also been found to be related to tumorigenesis. Several studies have shown that miR-144 is significantly down-regulated in a number of different cancer types, while it is widely considered to act as a tumor suppressor in glioblastoma. While the roles of miR-144 and HCMV in glioblastoma have been investigated separately, the effects of miR-144 have not been explored as intently in HCMV-positive glioblastoma cells. PURPOSE OF THE STUDY
Taking the previously mentioned facts into consideration, this study aimed to investigate the possible role of miR-144 in regulating the proliferation and migration of HCMV-negative and HCMV-positive U373MG glioblastoma cells. Over-expression of miR-144, through miRNA transfection experiments, led to significant suppression of cell growth and migration in both infected and non-infected cells, while miR-144 inhibition allowed cells to maintain their standard proliferative and migratory capacity. These results indicate that miR-144 does indeed act as a tumor suppressor in glioblastoma, by inhibiting cell proliferation and migration in both HCMV-negative and HCMV-positive U373MG cells.
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| Language |
Greek, English |
| Subject |
Ανθρώπινος κυτταρομεγαλοϊος |
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Γλοιοβλάστωμα |
| Issue date |
2023-04-05 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
424 |
Ο ρόλος του MIR-144 στον πολλαπλασιασμό και τη μετανάστευση μη μολυσμένων και μολυσμένων με τον ανθρώπινο κυτταρομεγαλοϊο (HCMV) κυττάρων γλοιοβλαστώματος
