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Identifier uch.biology.phd//1995athanasiadis
Title Δομική μελέτη της PVUII ενδονουκλεάσης
Alternative Title Structural study of the PVUII endonuclease
Author Αθανασιάδης, Αλέξανδρος Α
Thesis advisor Κοκκινίδης, Μιχαήλ
Abstract The PVUII endonouclease belong to the typeII bacterial restriction modification system PVUII of Proteus Vulgaris.We determind the sequence of the PVUII system and subsequently we developed a protocol for the purification of the protein to homogenity resulting in mg quantities of pure protein. We crystallized the PVUII endonouclease and determined the structure with the multiple isomorphous replacement method to a resolution of 2.4Α. The resulted model showeda topological similarity with the structure of the ECORV endonoulclease despite the lack of any sequence homology.This result indicates that possibly restriction enzymes can be categorized according to the position of scissile bond on DNA
Language Greek
Subject Ακτίνες Χ; Ενδονουκλεάσες; Ένζυμα; Καθαρισμός; Κρυσταλλογραφία; ΚΡΥΣΤΑΛΛΟΣΗ; ΠΕΡΙΟΡΙΣΤΙΚΟ
Issue date 1995-05-18
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
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