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Identifier

000428748

Title

Επίδραση της θερμοκρασίας ανάπτυξης, κατά το εμβρυϊκό στάδιο, στην παρεγκεφαλίδα του Danio rerio

Alternative Title

Effect of developmental temperature during embryonic stage on the cerebellum of Danio rerio

Author

Κούφαλης, Ευάγγελος Κ.

Thesis advisor

Κουμουνδούρος, Γεώργιος

Reviewer

Σιδηροπούλου, Κυριακή
Παυλίδης, Μιχαήλ

Abstract

Temperature is one of the most important factors, which can have a significant impact on ectotherm organisms. Temperature variations during early developmental stages of fish could affect their later stages. Temperature changes during the embryonic stage, has significant effects on the zebrafish cerebellum size. Fertilized eggs were exposed into three different developmental temperatures (TD = 24 °C, 28 °C, 32 °C) up to hatching and then all were maintained under common conditions (28 °C) till the end of metamorphosis. At the end of metamorphosis, the brain of juveniles of all treatments were removed and subjected to histological analysis. Using nissl staining, the area of three subregions of cerebellum was calculated: the anterior region (Valvula cerebelli, Va), the central region (Corpus Cerebelli, CCe) and the posterior region (Lobus Caudalis, LCa). The results showed that the area of these subregions was significantly increased in individuals that were initially exposed to 32 °C compared to those exposed to lower temperatures. Furthermore, the activation of c-Fos cells on the cerebellum of juveniles was studied as a response to prolonged swimming. Statistically significant differences between the groups did not noticed. Hence, our results indicate that even a short period of exposure to increased temperatures during the embryonic stage can affect later stages of zebrafish brain morphology, however it has no impact on the activation of c-Fos genes of cerebellum.

Language

Greek

Subject

Immunohistochemistry

Nissl staining

Phenotypic plasticity

Swimming

Zebrafish

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