Your browser does not support JavaScript!

Home    Διαλογή βιβλιοθήκης για την απομόνωση νέων ανθρώπινων γονιδίων HES και ανάλυση της έκφρασης των ήδη γνωστών σε κυτταρικές σειρές θηλαστικών  

Results - Details

Add to Basket
[Add to Basket]
Identifier uch.biology.msc//1998koumbanakis
Title Διαλογή βιβλιοθήκης για την απομόνωση νέων ανθρώπινων γονιδίων HES και ανάλυση της έκφρασης των ήδη γνωστών σε κυτταρικές σειρές θηλαστικών
Author Κουμπανάκης, Κωνσταντίνος Α
Thesis advisor Δελιδάκης, Χρήστος
Abstract Lateral inhibition is a kind of cell-cell communication and during development it is responcible for the generation of two or more different cell types from an initial population of cells with the same developmental potential. The transmembrane protein Notch plays a fundamental role in this process, acting as the receptor of an inhibitory signal that comes from a cell that has decided to differentiate towards its neighbouring cells, preventing them from adopting the same developmental fate and keeping them competent of receiving new inductive signals. In mammals, the Notch signal transduction pathway participates in several events during development, such as somitοgenesis, neurogenesis and myogenesis. In the present work we describe the effort to identificate new, unknown factors that participate in the signaling pathway, using the yeast two-hybrid system. We also describe the study of the effect of activated human receptors Notch 1 and 2 on the transcriptional levels of the endogenous genes of the HES family, via an RT-PCR based methodology. The HES gene family consists of transcriptional bHLH repressors, homologous to hairy/Enhancer of split genes of Drosophila melanogaster. These factors are some of the target genes of Notch signaling pathway and are considered as the last factors that participate in this signaling cascade.
Language Greek
Issue date 1998-11-19
Date available 1998-11-27
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Views 212

Digital Documents
No preview available

Download document
View document
Views : 1