E-Locus - Institutional Repository of the University of Crete - Ανάπτυξη μεθόδου προσδιορισμού των μεταβολιτών των φθαλικών εστέρων στα ούρα με χρήση υγρής χρωματογραφίας-δίδυμης φασματομετρίας μάζας με ιονισμό ηλεκτροψεκασμού

Home Ανάπτυξη μεθόδου προσδιορισμού των μεταβολιτών των φθαλικών εστέρων στα ούρα με χρήση υγρής χρωματογραφίας-δίδυμης φασματομετρίας μάζας με ιονισμό ηλεκτροψεκασμού

Results - Details

[Add to Basket]

Identifier

000368525

Title

Ανάπτυξη μεθόδου προσδιορισμού των μεταβολιτών των φθαλικών εστέρων στα ούρα με χρήση υγρής χρωματογραφίας-δίδυμης φασματομετρίας μάζας με ιονισμό ηλεκτροψεκασμού

Alternative Title

Quantitative detection of urinary phthalate metabolites in humans using liquid chromatography-electrospray ionization tandem mass spectrometry

Author

Μυριδάκης, Αντώνης Ιω.

Thesis advisor

Στεφάνου, Ευριπίδης

Abstract

The aim of this study was the development and application of an analytical method for the qualification and quantitation of phthalate ester metabolites in human urine samples using high performance liquid chromatography-tandem mass spectrometry with electrospray ionization (HPLC-ESI-MS/MS). Phthalates are commonly used as plasticizers (additives in plastics), they are found in many products and via these are spread to the environment in high amounts. The reason for the development of this method is its future use for the analysis and determination of phthalate metabolite levels in a large number of human urine samples for the first time in Greece and with only a few existing studies in Europe. The levels of phthalate metabolite concentrations are potential biomarkers of exposure in environmental epidemiology. Although it has been already shown in animals that exposure to phthalate esters causes defects, it is unclear whether human exposure results in adverse health effects. A sensitive, accurate, repeatable, selective method with high recoveries and blank concentrations less than limits of detection, was developed for the monitoring of six phthalate metabolites. The analysis is rapid and it can be performed in number of samples. The instrumental and method limits of detection and quantification are in the low ng/mL levels. Two interlaboratory comparisons for the method were performed and the results were almost identical. The method was applied in real samples for analyzing both free (12 samples) and total (24 samples) phthalate metabolite. The results were comparable with literature reports.

Language

Greek

Subject

Endocrine disruptros

Human urine

Metabolites

Phthalates

Υγρή χρωματογραφία

Φασματομετρία μάζας

Φθαλικοί εστέρες

Φθαλικοί μεταβολίτες

Issue date