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Identifier

000430828

Title

Self-assembled protein based materials and their applications

Alternative Title

Αυτο-οργανωμένα πρωτεϊνικά υλικά και εφαρμογές τους

Author

Νηστικάκης, Γεώργιος Σ.

Reviewer

Κοκκινίδης, Μιχαήλ
Μητράκη, Άννα

Abstract

This work focuses on the design, production and structural characterization of the recombinant protein Bio-S and its amyloid fibrils for use in tissue engineering, as biomaterials. Briefly, in this work the design and the heterologous expression of the recombinant protein Bio-S is shown, through PCR cloning, utilizing an already studied construct as a base. Also, the production and purification of the protein by affinity chromatography, as well as, its “re-naturation” and self-assembly into amyloid fibrils. Subsequently, chemical and optical characterization of these fibrils was conducted in order to ascertain their conformation and properties. Finally, the functionalization of the amyloid fibrils with gold metallization was attempted as application proof-of-principle and was observed by electron microscopy.

Language

English

Subject

Adenovirus type 2

Amyloid fibrils

Biomaterials

Protein scaffolds

Self-assembly

Αδενοϊός τύπου 2

Αμυλοειδή ινίδια

Αυτο-οργάνωση

Βιουλικά