E-Locus - Institutional Repository of the University of Crete - Ο ρόλος του σηματοδοτικού μονοπατιού CD40 στον θάνατο καρκινικών κυττάρων.

Home Ο ρόλος του σηματοδοτικού μονοπατιού CD40 στον θάνατο καρκινικών κυττάρων.

Results - Details

[Add to Basket]

Identifier

000374173

Title

Ο ρόλος του σηματοδοτικού μονοπατιού CD40 στον θάνατο καρκινικών κυττάρων.

Alternative Title

The CD40 pathway in malignant cell death

Author

Ζαραγκούλιας, Ανδρέας

Thesis advisor

Ηλιόπουλος, Αριστείδης

Reviewer

Σουρβίνος, Γεώργιος

Abstract

CD40 is a member of tumor necrosis factor receptor (TNFR) superfamily that is expressed in a variety of cancers such as leukaemias, lymphomas and carcinomas. Different experimental and clinical studies till day suggest that CD40 pathway can be useful in cancer therapy. At this point, in the present work we studied the liposome delivery of CD40L gene in carcinoma cells. The results was not successful, but this doesn't mean that liposomes as a delivery system is a bad strategy. Secondly, we evaluated the effect of Akt knock-down in cell survival of carcinoma cells and we saw that maybe in the future we could use the Akt knock-down together with CD40L as a combinatorial therapeutic approach. Moreover, we examined in vitro the direct anti-tumor effect of transmembrane CD40L and of secreted trimeric CD40L, and the results were promising because there was an advantage of secreted trimeric CD40L, as it doesn't interact only nearby with some cells, but also it can interact with many cells in the whole tumor microenviroment. Finally, we evaluated the DC maturation following adenovirus transduction with CD40L, as DCs could have direct anti-tumor effect by CD40L, and also indirect immune-mediated anti-tumor effect. In conclusion, all the above studies just put some first bases for farther in vitro and mostly in vivo experiments, that will elucidate the CD40 pathway role in cancer treatment.

Language

Greek

Subject

CD40 pathway

CD40 μονοπάτι

Cancer

Malignant cell death

Pathology

Καρκίνος

Καρκινικά κύτταρα

Issue date

2010-12-14