E-Locus - Institutional Repository of the University of Crete - Μελέτη του ρόλου των γονιδίων P73,P53 και P21 στη ρύθμιση του κυτταρικού κύκλου σε υπερπλασίες και κακοήθειες του προστάτη

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Identifier

000378469

Title

Μελέτη του ρόλου των γονιδίων P73,P53 και P21 στη ρύθμιση του κυτταρικού κύκλου σε υπερπλασίες και κακοήθειες του προστάτη

Alternative Title

Study of the role of genes P73 ,P53 and P21 in regulating the cell cycle in prostate cancer and benign hyperplasia

Author

Λιανός, Εμμανουήλ

Thesis advisor

Σπαντίδος, Δ.

Reviewer

Σοφράς, Φ
Σουρβίνος, Γ.

Abstract

Introduction: P53 protein is important for growth suppression and induction of apoptosis. p73, a recently discovered p53 homolog, undergoes complex alternative splicing and produces several isoforms who differ in their C-terminal, as well as in their potential to substitute p53 functions. Material and Methods: We investigated the transcriptional levels of p53-pathway genes, p53, p73, MDM2 and p21WAF1/CIP1 in 15 prostate carcinomas, 21 benign prostate hyperplasias (12 with acute or chronic inflammation) and 8 normal prostate tissues by means of semi-quantative RT-PCR, using α2-microglobulin as internal control. Moreover we used an additional primer set to detect the levels of p73 isoforms with or without exon 13. Results: The ratio of exon 13 transcripts/total p73 mRNAs was significantly increased 6 to 8-fold in both tumors (80% of cases) and hyperplasias (52% of cases). MDM2 and p53 transcriptional levels were decreased 4-fold in 67% of cancer and 45% of benign lesions without inflammation, while they were increased 6-fold in 75% of inflammatory hyperplasias. p21WAF1/CIP1 levels were relatively constant. Conclusion: Our data suggest that alternative splicing shift of p73 in benign prostate hyperplasia and prostate cancer compared to normal controls could be a key element in the development of these diseases.

Language

Greek

Subject

Benign prostate hyperplasia

Cell cycle

Exon 13 P73

Exon2 P73

P21 gene

P53 gene

P73 gene

Prostate cancer

Γονίδιο P53

Γονίδιο P73

Γονίδιο Ρ21

Εξώνιο 13 Ρ73

Εξώνιο 2 Ρ73

Καλοήθη υπερτροφία προστάτου

Κυτταρικός κύκλος

Issue date

2012-12-11

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses

Views

279

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