E-Locus - Ιδρυματικό Καταθετήριο Πανεπιστημίου Κρήτης - Investigating cMaf as a key driver of autoimmunity

Αρχική Investigating cMaf as a key driver of autoimmunity

Αποτελέσματα - Λεπτομέρειες

[Προσθήκη στο καλάθι]

Κωδικός Πόρου

000468888

Τίτλος

Investigating cMaf as a key driver of autoimmunity

Άλλος τίτλος

Διερευνώντας τον ρόλο του cMaf ως ρυθμιστή της αυτοανοσίας

Συγγραφέας

Μωρρές, Ελευθέριος Ι.

Σύμβουλος διατριβής

Σπηλιανάκης, Χαράλαμπος

Μέλος κριτικής επιτροπής

Μπέρτσιας, Γεώργιος
Lavigne, Matthieu

Περίληψη

Special AT-rich Sequence-Binding Protein 1 (SATB1) is a lineage specific transcription regulator which plays a pivotal role in orchestrating the spatiotemporal development of T cells. Although it is known that SATB1 deletion is associated with autoimmunity, the precise mechanisms underlying this phenotype are poorly understood. In this study we investigate the drivers of the autoimmunity that SATB1 deletion causes and how it is reversed upon simultaneous deletion of SATB1 and Bach1. We performed single cell transcriptomic analysis, and identified a newly formed cluster, characterized by the expression of cMaf. Furthermore, using an array of bioinformatic tools we discovered that this unique cluster exhibits an activated phenotype, seemingly regulated by cMaf. Additionally, immunofluorescence experiments unveiled disrupted spleen morphology in SATB1-depleted animals, a disruption absent in animals with combined SATB1 and Bach1 depletion. Altogether, our findings highlight cMaf a mediator of autoimmunity, and strengthen the notion that double SATB1 and BACH1 depletion rescues the autoimmune phenotype.

Φυσική περιγραφή

51 σ. : πίν., σχήμ., εικ. (μερ. εγχρ.) ; 30 εκ.

Γλώσσα

Αγγλικά

Θέμα

Bach1

ISP cells

Satb1

Sc-RNA seq

Ημερομηνία έκδοσης

2024-11-29

Συλλογή