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| Identifier |
000402874 |
| Title |
Imaging and quantification of lipid stores in adipose and muscular tissue in Caenorhabditis elegans samples via nonlinear microscopy |
| Alternative Title |
Απεικόνιση και ποσοτικοποίηση λιπιδικών εναποθέσεων σε λιπώδη και μυικό ιστό του νηματοειδούς Caenorhabditis elegans μέσω μη γραμμικής μικροσκοπίας |
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Author
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Πετανίδου, Βαρβάρα Α.
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Thesis advisor
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Φωτάκης, Κωνσταντίνος
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Reviewer
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Ταβερναράκης, Νεκτάριος
Αθανασάκη, Ειρήνη
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| Abstract |
The investigation of molecular mechanisms that regulate lipid storage and its metabolism through non-destructive imaging techniques can provide valuable and significant information for preventing obesity that is related with a sequence of very serious pathological situations such as cardiovascular disorders and diabetes type II.
In current study, nematode Caenorhabditis elegans (C. elegans) has been used as a model organism. The accomplishment of non-linear imaging measurements of second (SHG), third harmonic (THG) generation and multiphoton fluorescence (MPEF) provided for the first time in details the morphology, structure and lipid depositions in samples at microscopic level.
By employing these non-destructive techniques, imaging and monitoring the lipid accumulation has been achieved in adipose and non-adipose (ectopic fat) tissue in the region of intestine and pharynx respectively in non labelled wild type and mutant animals. The quantification of the collected non-linear signals of third harmonic generation reveals the correlation between lipid depositions and aging of the sample. It was also shown that a significant increase in lipids in the intestine region (adipose fat) during developmental stages of C. elegans followed by a decrease during its adult lifespan. Moreover, it has been detected an increase of lipid depositions in the pharynx region (ectopic fat) during aging of the biological samples.
These results could afford remarkable and profound information for the relation between aging process and lipotoxicity in the model organism C. elegans. Additionally, they prove that third harmonic generation can act as a novel, reliable fast and non-invasive tool for providing new insights in lipid biology research.
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| Language |
English |
| Subject |
Adipose fat |
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C. elegans |
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Ectopic fat |
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Third harmonic generation |
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Έκτοπο λίπος |
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Λίπος μυών |
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Λιπίδια |
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Μη γραμμική μικροσκοπία |
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Νηματοειδές |
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Ποσοτικοποίηση λιπιδίων |
| Issue date |
2016-11-18 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Physics--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
491 |
Imaging and quantification of lipid stores in adipose and muscular tissue in Caenorhabditis elegans samples via nonlinear microscopy
