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Identifier

000369243

Title

Στόχευση προπρωτεϊνών στην μεμβράνη με την βοήθεια σαπερονών

Alternative Title

Chaperone assisted preprotein targeting to the membrane

Author

Εμμανουηλίδης, Λεωνίδας

Thesis advisor

Οικονόμου, Α

Abstract

In this thesis we studied chaperone assisted preprotein targeting to the membrane. Using as substrate the preprotein, alkaline phosphatase, we found the necessary characteristics for the binding of chaperones SecB and Trigger factor. Our method involves size exclusion chromatography connected with MALLS / QELS, which proved that it is necessary for substrate to be in the molten globule state in order to bind with both chaperones. Furthermore, the mechanism by which chaperones recognize the proprotein found to be different in every case. Trigger factor rather may recognize directly or indirectly the existence of signal peptide, whereas SecB forms a stable complex even with the mature domain of alkaline phosphatase. Additionally, we have shown that SecB is able to transfer preprotein to the ATPase SecA in solution. Thus, we propose a mechanism for chaperone assisted preprotein targeting to the membrane.

Language

Greek

Subject

SecA

SecB

Trogger Factor

Issue date

2011-11-18

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

Views

242

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