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Identifier

000334845

Title

Πρόβλεψη και πειραματική επιβεβαίωση mRNA-miRNA αλληλεπιδράσεων

Alternative Title

Prediction and experimental verification of mRNA-miRNA interactions

Author

Καραθανάσης, Νέστορας

Thesis advisor

Πoϊράζη, Παναγιώτα
Καραγωγέως, Δόμνα

Abstract

Previous reports have identified 154 mRNAs, which are over-expressed in the dendritic area of the rat hippocampal CA1 region (8). Since most synaptic contacts occur in this area, it is possible that several of these mRNAs are directly involved in synaptic plasticity. Given that local protein synthesis in the dendrites has been shown to underlie certain forms of synaptic plasticity (reviewed in (9)), plus the fact that P-bodies are present in dendrites of rat hippocampal neurons we are testing the hypothesis that some of these mRNAs are being regulated by miRNAs to ensure that translation occurs only under desired conditions, in this case in response to plasticity-inducing stimuli. A number of five genes, GRIA1, GRIA2, GRIA3, KCNA1, CAMK2A known to play a role in LTP/LTD and whose mRNAs are present in the dendrites of mouse hippocampal neurons were selected to be examined as possible miRNA targets (1, 2). Their mRNAs were scanned using computational approaches and mRNA-miRNA interactions were predicted. The programs Pictar, TargetScan, Miranda and PITA along with a heuristic set of filtering rules were used for this analysis. The rules were developed in order to constrain the large number of predicted interactions that often result from target prediction programs. The reasoning for the program selection and associated filtering rules is based on the available biological information regarding the features of miRNA-mRNA interactions. The best scoring interaction was selected for further experimental verification. It was shown that mir-124 has at least one functional seed site in gria2’s 3’UTR.

Language

Greek

Subject

Miranda

P-bodies

PITA

PicTar

RNA, Messenger

TargetScan

Issue date

2008-12-04

Collection