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| Identifier |
uch.biology.msc//2001asoutis |
| Title |
Ανεύρεση και βιοχημικός χαρακτηρισμός δυο βιοενεργών λεκτινών στην αιμόλεμφο του θαλάσσιου καβουριού Calappa Granulata-προοπτικές ανίχνευσης των αντίστοιχων γονιδίων |
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Author
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Ασούτης - Διδάρας, Νικόλαος
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| Abstract |
Lectins are proteins that recognize saccharide structures (Carbohydrates, Glycoproteins, Glycolipids). They agglutinate cells (eucaryotics, procaryotics) or have an effect on their biology.Important applications of lectins are found within Biotechnology and Biomedicine. The Purification, Characterization and Use of lectins constitute an important research field. Crustaceans are a source of lectins with prototype specificities on Carbohydrate binding. In this master thesis we report the Identification and Biochemical Characterization of two Lectins from the hemolymph of the marine crab Calappa granulata. The first lectin agglutinates rabbit red blood cells stronger than human's and is highly specific for α-galactosides; it doesn't recognize N-acetyl-galactoside or glucose. This is a prototype specificity for a Crustacean lectin. It was purified after absorption on P-nitro-phenyl-α-D-galactoside-sepharose 6B, or asialofetuin-sepharose 4B and desorption with EDTA or α-lactose respectively; it is composed of 28 + 2 kDa subunits and is glycosylated. The other lectin recognizes sialic acids, N-acetyl-glucosamine, N-acetyl-galactosamine and strongly agglutinates horse red blood cells. It was purified after absorption on N-acetyl-glucosamine-sepharose 6B and desorption with N-acetyl-glucosamine. The protein is composed of 39 + 2 kDa and 42 + 2 kDa subunits and is also glycosylated. To detect the genes of these two lectins a cDNA library has been made from various tissues of Calappa granulata.
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| Language |
Greek |
| Issue date |
2001-03-22 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
242 |
Ανεύρεση και βιοχημικός χαρακτηρισμός δυο βιοενεργών λεκτινών στην αιμόλεμφο του θαλάσσιου καβουριού Calappa Granulata-προοπτικές ανίχνευσης των αντίστοιχων γονιδίων
