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Identifier

000367064

Title

Μελέτη της γονιδιακής έκφρασης στη συμβιωτική σχέση Wolbachia-Drosophila

Alternative Title

Study of gene expression in the Wolbachia-Drosophila symbiotic association

Author

Παπαφωτίου, Γιώργος

Thesis advisor

Σαββάκης, Χαράλαμπος

Thesis advisor

Μπούρτζης Κωνσταντίνος

Abstract

Wolbachia are obligative intracellular bacteria that infect a great number of species of two invertebrate phyla and nematode worms. The relationship between the bacteria and the host can range from parasitism to mutualism. The most common effects of the infection with Wolbachia are those of reproductive parasitism, which is the manipulation of the host reproduction to the benefit of the bacteria. Despite the fact that the phenotypes are well characterized the underlying molecular mechanisms are not known. A number of Wolbachia strains which infect Drosophila, can induce cytoplasmic incompatibility, which is expressed as embryo mortality in crosses of infected males to uninfected females. Bacterial ANK genes, encoding for ankyrin homology domain proteins, have been suggested as candidates for involvement in CI and/or other aspects of the interaction. In order to identify genes, which are involved in the crosstalk between the bacteria and the host, this study, focused on two main points. Firstly, the genome-wide study of Drosophila gene expression in a number of tissues and stages in the presence of Wolbachia, and secondly the study of Wolbachia ANK gene expression and ANK protein localization in a number of symbiotic associations. The main findings of this study are a) Wolbachia do not affect host gene expression to a detectable level in whole animals and tissues, b) ANK genes WD0438 and WD1213 from the wMel Wolbachia strain are regulated in a tissue-specific way in D. melanogaster and in D. simulans and c) the regulation of the above genes does not seem to be correlated with the expression of cytoplasmic incompatibility.

Language

Greek

Subject

Cytoplasmic Incompatibility

Κυτταροπλασματική ασυμβατότητα

Issue date

2011-07-15

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses

Type of Work--Doctoral theses

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230

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